等离激元增强型多层纳米核壳结构红外荧光探针及其对乳腺癌细胞的定位标记

项目名称： 等离激元增强型多层纳米核壳结构红外荧光探针及其对乳腺癌细胞的定位标记

项目编号： No.51272246

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 许小亮

作者单位： 中国科学技术大学

项目金额： 80万元

中文摘要： 量子点荧光标记已经在生物医学领域取得了令人瞩目的成绩，红外量子点由于对生物组织的穿透性强而备受关注。为了显著提高量子点的发光强度，使之更加适用于活体标记与临床治疗，本项目提出一种新型的多层纳米金属核-壳结构加量子点构成的等离激元增强型荧光探针，其结构为"M1核@SiO2包覆量子点@M2壳"，其中M1为内层纳米金或银球，M2为最外纳米金壳层。在外加光场作用下，核与壳产生的等离激元杂化能量共振效应，有高能ω-+ 耦合和低能ω- - 两种耦合方式，进而在短波端与长波端各产生一个等离激元共振吸收峰，峰位可通过调节多层结构的尺度而加以调整，实现对入射光和出射光同时增强的效果。该探针可望实现显著提高发光强度及提高组织穿透能力的目的。使受标记的乳腺癌组织、前哨淋巴结和转移淋巴结在外部光源的激发下，产生各色能穿透体表组织的可见光和红外光，以期对乳腺癌浸润范围、前哨淋巴结及转移淋巴结进行示踪定位应用提供基础

中文关键词： 等离激元；红外；荧光；乳腺癌；标记

英文摘要： Study of plasmonic enhanced multi core-shell nanostructured infrared fluorescence probes and its labelling for breast cancer cells 1.Abstract: QDs fluorescence labeling has been obtained a significant achievement in biomedical fields, the infrared QDs in which are special under a highlight due to their excellent penetrability to the organisms. In order to notably increase the emission intensity from the QDs, to be more appropriate for the in vivo marker and clinical, we now present a new multilayer nanometal core-shell plus QDs structured plasmonic enhanced fluorescence probe. The construction of which namely "M1 @ SiO2 cladding QDs @ M2", whell M1 is Ag or Au nanosphere inner core, M2 is Au outermost nanoshell. Under the irradiation of the light field, the metallic core and shell could create a plasmonic hybridization energy resonance effects, have a higher energy coupling ω-+ and lower energy coupling ω- - , corresponding to the lower and higher wavelengths'plasmonic resonance absorption peaks. And the peak positions could be adjusted by regulating the scale of the multilayer structure, and therefore realize to enhance the intensity of the excitation light and the emission light, simultaneously. The new plasmonic enhanced probe is expected to significantly increase the fluorescence intensity and the light p

英文关键词： plasmonic；infrared；fluorescence；breast cancer；labelling