草鱼肠道黏膜屏障的损伤与益生芽孢杆菌的保护机制

项目名称： 草鱼肠道黏膜屏障的损伤与益生芽孢杆菌的保护机制

项目编号： No.31472310

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 吴志新

作者单位： 华中农业大学

项目金额： 85万元

中文摘要： 肠道疾病是当前水产动物重要病害，肠道黏膜屏障的完整对鱼类抵御病原生物及其他有害物质的入侵有重要作用，对肠道黏膜屏障功能的维持和保护是保持动物体健康的重要手段。鱼类肠道黏膜屏障损伤与益生芽孢杆菌的修复和保护机制，目前并不清楚。本项目围绕草鱼肠道黏膜屏障的损伤和益生芽孢杆菌对黏膜屏障保护的分子基础与作用机制，通过利用病原性嗜水气单胞菌、TNF-α、益生芽孢杆菌分别处理或共处理草鱼及其肠上皮培养细胞，采用免疫荧光、Western blot、ELISA、荧光定量PCR等方法，从肠黏膜上皮细胞的结构、通透性、紧密连接相关分子、细胞凋亡因子和凋亡通路信号因子，以及炎性因子及抗氧化应激因子等方面，揭示草鱼肠道黏膜屏障损伤机理及损伤修复过程中益生芽孢杆菌的作用及其保护机理，研究结果为改善鱼类肠道炎性疾病和阻断病原通过肠道黏膜的入侵途径提供新的思路。

中文关键词： 草鱼；肠道黏膜屏障；损伤；保护；益生芽孢杆菌

英文摘要： Intestinal disease is one of the most important diseases in aquaculture. Nevertheless, the integrity of intestinal mucosal barrier plays an important role in protection of fish to resist the invasion of pathogens and hazardous substances. Thus, maintaining the integrity of intestinal mucosal barrier is a significant means to keep animal healthy. Until now, the damage of intestinal mucosal barrier by pathogens infection and the repairing and protection mechanism of probiotic Bacillus is still unknown in the grass carp(Ctenopharyngodon idellus). In this project, we focuses on the mechanism of damage the intestinal mucosal barrier and protection of probiotic Bacillus in grass carp. Aeromonas hydrophila、TNF-α and probiotic Bacillus or alone was used to treat grass carp and primary cultured intestinal epithelium cells of grass carp, respectively. Then the structure and permeability of intestinal mucosal epithelial cells, and their the expression profile of tight junction related molecules, apoptosis related molecules, and inflammation and oxidation injury related molecules were analyzed by QPCR、western blot、and ELISA. The Aim is to reveal the impact of Aeromonas hydrophila on the intestinal mucosal barrier and its repairing and protection mechanism of probiotic Bacillus in grass carp, which could provide new sight for treating the fish intestinal inflammatory diseases and blocking the invasion pathway via the intestinal mucosa.

英文关键词： grass carp;intestinal mucosal barrier;damage;protection;probiotic Bacillus