硒化修饰提高中药多糖的增强免疫和抗氧化活性及其机理研究

项目名称： 硒化修饰提高中药多糖的增强免疫和抗氧化活性及其机理研究

项目编号： No.31272596

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 胡元亮

作者单位： 南京农业大学

项目金额： 77万元

中文摘要： 本项目拟在前期研究证明硫酸化修饰能显著提高中药多糖的抗病毒和增强免疫活性、前期预研证明硒化修饰能显著提高中药多糖的抗氧化和增强免疫活性的基础上，选择10种中药多糖为研究对象，分别从相应中药中提取总多糖和分级多糖；以增强免疫活性和抗氧化活性为指标，追踪活性部位；建立各多糖的硒化修饰方法，对各多糖活性部位进行硒化修饰；运用药理学、免疫学、分子生物学等现代技术，系统比较修饰前、后各多糖的活性变化；筛选出各多糖中活性最强的硒化多糖，并从分子、细胞和临床学方面阐明其作用机制；最后进行总体比较，筛选出活性最强的硒化多糖，以发现多糖类增强免疫和抗氧化活性先导化合物，为研制开发高效、安全、具有自主知识产权的新型免疫增强剂和饲料添加剂奠定基础。本研究不仅对开发利用植物资源、开辟新药源、保障中药的可持续发展具有重要的战略意义，而且对研制开发新兽药、提高动物疫病的防控效果、促进国民经济的发展具有广阔的应用前景。

中文关键词： 中药多糖；硒化修饰；增强免疫活性；抗氧化活性；作用机理

英文摘要： On the basis of the previous and previous preliminary researchs which confirmed that sulfating modification could significantly enhance the antiviral and immune-enhancing activities and selenizing modification could significantly enhance the anti-oxidative and immune-enhancing activities of Chinese herbal medicinal polysaccharides, this project will carry out following researches: Firstly, ten kinds of Chinese herbal medicinal polysaccharides will be selected as research object and the total and fractional polysaccharides of each kind of polysaccharide will be extracted respectively from the corresponding Chinese herbal medicines. Secondly, the active sites of every kind of polysaccharide will be traced taking the immune-enhancing and anti-oxidative activities as the index. Thirdly, selenizing modification methods for each kind of polysaccharide will be established and the active sites of each kind of polysaccharide will be modified, respectively. Fourthly, the activity changes of each kind of polysaccharide before and after modification will be compared systematically by means of modern technique on pharmacology, immunology, molecular biology and so on. Fifthly, the selenizing polysaccharides with strongest activity in every kind of polysaccharide will be picked out and their mechanisms of action will be clarit

英文关键词： Chinese herbal medicinal polysaccharide；selenylation modification；immune-enhancing activity；antioxidant activity；action mechanism