高山离子芥DREB2A转录因子对冷响应基因的调控机制研究

项目名称： 高山离子芥DREB2A转录因子对冷响应基因的调控机制研究

项目编号： No.31301078

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 岳修乐

作者单位： 兰州大学

项目金额： 23万元

中文摘要： 低温冻害是一种重大的自然灾害,它严重影响着农作物的生长发育、产量和品质。提高农作物的低温耐受力是亟待解决的科学问题。前人的研究表明，冷响应基因的表达调控主要有ABA依赖型和ABA非依赖型（DREB1/CBFs）调控通路，然而，目前DREB1/CBFs型调控通路的研究理论不足以支撑具有天然耐冻植物的胁迫表型。本课题以天然抗冻的冰缘植物高山离子芥为研究材料，以通过转录组测序和消减杂交得到的唯一一个DRE/CRT类冷响应转录因子DREB2A为研究对象，利用荧光定量PCR技术研究其表达谱，揭示其低温响应模式；通过基因沉默和过表达来研究其在冷冻胁迫中发挥的作用,揭示其作用机制；利用转录组测序来研究受其调控的下游冷响应基因,阐明其转录调控特征及获得起关键抗冻作用的候选基因。以上研究可为揭示高山离子芥的冷响应调控机制奠定基础，为农作物的耐冻性改造和寒区的生态恢复提供理论依据和技术支撑。

中文关键词： 高山离子芥；低温胁迫；DREB2A；转录因子；

英文摘要： The low-temperature injury is one of the major disasters to crops, it adversely affects their growth, development, yield and quality. To improve crops' low temprature tolerance is an urgent task for the scientists.Previous research shows that the cold responsive genes are regulated by mainly two pathways-ABA dependent and ABA independent(DREB1/CBFs)pathway. However, the molecular regulation theory of DREB1/CBFs pathway is insufficient to support the the phynotype of the natural cold tolerant plants.This project take Chorispora bungeana-an alpine subnival plant as research material,focusing on revealing the regulation mechanism of DREB2A transcription factor which is found by RNA sequence and suppression subtractive hybridization(SSH) under cold stress.We aim to reveal it's function by gene over-expression and gene silence, to find the cold responsive genes regulated by DREB2A through RNA sequence and real-time PCR,and also to indicate the characteristics of its transcriptional regulation and get the key candidate genes to resist cold stress based on comprehensive analysis of the the downstream cold response genes. The above study will lay a foundation for revealing the transcription regulation mechanism of Chorispora bungeana under cold stress, and provide a theoretical basis and technical support for the crop

英文关键词： Chorispora bungeana；cold stress；DREB2A；transcript factor；