单胺类神经递质及其代谢物活体原位采样、在线印迹富集、实时检测新方法研究

项目名称： 单胺类神经递质及其代谢物活体原位采样、在线印迹富集、实时检测新方法研究

项目编号： No.91232703

项目类型： 重大研究计划

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 李攻科

作者单位： 中山大学

项目金额： 70万元

中文摘要： 单胺类神经递质是一类具有广泛生物学活性的物质，抑郁症等神经系统疾病与单胺类神经递质的异常有关。因此，生物活体中单胺类神经递质及其代谢产物原位实时在线分析检测技术及其方法研究成为神经生物化学及分析化学的前沿课题。本项目集成微透析活体采样技术、印迹微萃取在线分离富集技术及快速高效的色谱及光谱检测技术于一体，研制单胺类神经递质分子印迹微萃取介质；设计生物活体中单胺类神经递质微透析活体原位采样-印迹微萃取联用接口，构建实时动态监测装置；研究生物活体中单胺类神经递质及其代谢产物实时在线分析检测新方法；探索实时在线分析检测新技术在抑郁症神经生化机制、情感和记忆过程研究中的应用。研究成果将为抑郁症等疾病的产生及诊疗研究提供科学方法手段，将促进生理学、病理学及药理学的研究，为从分子水平上开展情感与记忆过程研究提供关键方法与技术。

中文关键词： 单胺类神经递质；代谢产物；在线分离富集；在线分析；生物样品

英文摘要： Monoamine neurotransmitter is one of the most important neural transmission medium with broad biological activities. Some nervous system diseases such as depression are always associated with the abnormal level of monoamine neurotransmitter. Therefore, in vivo monitoring techniques for the determination of monoamine neurotransmitters and their metabolites in living organisms have become advanced topics in nerve biochemistry and analytical chemistry. In this project, in vivo microdialysis sampling technique,online imprinted microextraction sample preconcentration and separation technique coupled with the rapid and efficient chromatographic and spectroscopic detection techniques are developed. It mainly involve several aspects including developing monoamine neurotransmitters molecularly imprinted microextraction medium, designing an interface for coupling of in vivo microdialysis sampling and the simultaneously imprinted microextraction, building the real-time dynamic monitoring device for monoamine neurotransmitters, exploring a new online analytical method for determination of the monoamine neurotransmitters and their metabolites in living organisms and its application in neural biochemical mechanism of depression, in emotion and memory processes study. This research work will provide scientific methods for t

英文关键词： Monoamine neurotransmitter；metabolite；online separation and enrichment；online analysis；biological sample