基于三维聚焦技术的微流控芯片-NANO电喷雾质谱新方法

项目名称： 基于三维聚焦技术的微流控芯片-NANO电喷雾质谱新方法

项目编号： No.21205044

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 熊博

作者单位： 华中师范大学

项目金额： 25万元

中文摘要： 随着微流控芯片-NANO电喷雾质谱方法受到越来越多的关注，如何抑制锥形电喷雾急剧扩散所导致的进样损耗成为颇具实际意义的问题。现有研究侧重于制备各种喷雾口以缩小整体电喷雾。基于这种思路进行芯片制作，难度明显增大。本研究从控制总液流中的样品分布入手，调控锥形电喷雾中的待测物分布，使样品尽可能多的进入质谱。基于此思路，既可降低芯片制作难度，又能在加入挥发性溶剂以稳定电喷雾的同时，对样品液流进行三维聚焦控制，进而抑制进样损耗以提升灵敏度。本研究拟在优化水平方向和垂直方向二维聚焦芯片的基础上，制备三维聚焦芯片，并基于该芯片对样品微液流进行三维聚焦控制和电喷雾电离，以实现芯片和质谱的在线串联，从而建立基于三维聚焦技术的微流控芯片-NANO电喷雾质谱高灵敏度分析新方法。最后，将该方法应用于检测磷酸化多肽并评价其检测限。通过本课题的研究，以期为基于芯片平台对样品中的痕量组分进行在线结构定性打下良好的基础。

中文关键词： 三维聚焦；微流控芯片；NANO电喷雾质谱；；

英文摘要： With more and more attentions for microfluidic chips coupled with the nanoelectrospray ionization mass spectrometry, it is of great practical significance to depress the injection losses from the serious diffusion of the electrospray cone. Current researches focused on the preparation of various spray orfices to compress the overall spray. Following this idea, the difficulty to prepare microfluidic chips increased obviously. In this study, by controlling the distribution of the sample micro flow in the overall flow, the analyte distribution in the electrospray cone will be regulated. In this way, analytes will be injected into the mass spectrometer as much as possible, and thus the injection losses will be reduced. Following the new idea, the difficulty to prepare microfluidic chips will be decreased. In addition, it is probable to realize 3D hydrodynamic focusing for the sample flow to depress preceding injection losses and improve its detection sensitivity, as well as to add volatile solvents in order to stabilize the overall electrospray. Based on the optimization for 2D hydrodynamic focusing chips in both vertical and horizontal directions, 3D hydrodynamic focusing chips will be prepared and utilized to realize 3D hydrodynamic focusing and the electrospray ionization for the sample micro flow, based on which

英文关键词： three-dimensional hydrodynamic focusing；microfluidic chip；nanoelectrospray ionization mass spectrometry；；