棉酚旋光异构体在泌乳奶牛体内转移规律及其代谢组研究

项目名称： 棉酚旋光异构体在泌乳奶牛体内转移规律及其代谢组研究

项目编号： No.31472123

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 张军民

作者单位： 中国农业科学院北京畜牧兽医研究所

项目金额： 88万元

中文摘要： 全棉籽中游离棉酚约占总棉酚的90%，而棉酚有左旋和右旋棉酚两种旋光异构体，左旋棉酚有更强的生物毒性。当棉酚超过瘤胃解毒能力，可影响奶牛生产性能和繁殖能力甚至造成奶牛死亡。棉酚旋光异构体在奶牛体内转移规律、棉酚毒性影响泌乳牛代谢组变化规律等科学问题尚不清楚。因此，拟通过奶牛饲养试验，利用LC-MS法测定奶牛血浆、粪、尿和乳中棉酚旋光异构体含量，研究日粮中棉酚旋光异构体向奶牛体内转移规律；采用持续动态人工瘤胃法，利用LC-MS法测定瘤胃液中棉酚旋光异构体含量，研究棉酚旋光异构体在瘤胃内代谢规律；通过奶牛十二指肠瘘管灌注试验，利用LC-MS法测定奶牛血浆、粪、尿和乳中棉酚旋光异构体含量，研究过瘤胃棉酚旋光异构体向奶牛体内转移规律；采用NMR、LC-MS法研究奶牛代谢组，探索棉酚毒性影响奶牛代谢组变化的规律和潜在的生物标志物。研究结果将为棉酚在奶牛体内转移及其对奶牛毒性作用提供理论依据。

中文关键词： 棉酚旋光异构体；泌乳牛；转移规律；代谢组

英文摘要： Free gossypol in cottonseed accounts for about 90% of total gossypol. There are two enantiomers of gossypol: (+)isomer and (-)isomer. (-)isomer is of more biological toxicity. Gossypol may affect production and productivity of cows, and may even cause death when too much gossypol is included in the diet. It is not clear how gossypol is transferred from diet to milk and how gossypol affects the metabolome of the cow. In relationto these problems, a feeding trial will be done using lactating cows to measure gossypol enantiomers concentration in blood, faeces, urine and milk as. Transfer regularity of gossypol enantiomers to the cow in vivo will be studied. And by using the continuous dynamic artificial rumen method, gossypol enantiomers concentration of rumen fluid will be determined by LC-MS, and the metabolic pathway of gossypol enantiomers will be investigated. Perfusion of gossypol through duodenal fistula will be performed in cows to demonstrate distributions of two different gossypol enantiomers, and gossypol enantiomers concentration in` blood, faeces, urine and milk will be determined by LC-MS. Metabolites in the cow's blood and urine will be determined by NMR and LC-MS, to explore the mechanism of how gossypol affectes metabolome of the cow and to identify its potential biological marker. The theoretical basis for regularity of gossypol transferred into the cow in vivo and effect of gossypol toxicity on cows will be determineded.

英文关键词： gossypol enantiomers;lactating dairy cows;transfer regularity;metabolome