背俞指针疗法对GERD大鼠SCF-c-kit-ICC/PLC-Ca2+-PKC/CaM信号通路与任督二脉穴位皮温的影响

项目名称： 背俞指针疗法对GERD大鼠SCF-c-kit-ICC/PLC-Ca2+-PKC/CaM信号通路与任督二脉穴位皮温的影响

项目编号： No.81460723

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 谢胜

作者单位： 广西中医药大学

项目金额： 48万元

中文摘要： 胃食管反流病(GERD)发病率呈上升趋势，单纯西医药治疗尚有不少困惑。中医背俞指针疗法疗效肯定，但机制不明。我们提出了任督二脉经气升降交会失衡是脾胃升降失衡致病的病机本质的假说，且已证明任督二脉经气升降交会失衡与上消化道动力障碍密切相关。新近发现SCF/c-kit-ICC可通过Ca2+-CaM-MLCK通路与PLC-Ca2+-PKC通路调控胃食管平滑肌舒缩功能。因而，我们认为本疗法很可能通过改善任督二脉经气交会失衡，进而影响PLC-Ca2+-PKC与SCF/c-kit-ICC起搏并增强Ca2+-CaM-MLCK通路调控平滑肌的舒缩功能。本项目采用背俞指针疗法干预GERD模型大鼠，探讨其对任督二脉主要穴位皮温、PLC-Ca2+-PKC与ICC相关信号通路的影响及其相关性，深入研究GERD经络-分子相关病机之本质，进一步证明前期假说的科学性，为该疗法在消化道动力障碍疾病的应用提供科学依据。

中文关键词： 脾胃；背俞指针疗法；胃食管反流病；信号通路；穴位皮温

英文摘要： In recent years, the incidence of Gastroesophageal reflux disease（GERD）is rising and the Western medical treatment alone still show a lot of confusion. Beishu Acupoint Finger Acupuncture Therapy(BAFAT) of traditional Chinese medicine is effective, but the mechanism is unknown. we have concluded that Functional unbalance of hoisting and delivering in Ren-Du meridian should be the pathogenic essence, as the spleen and stomach's hoisting functional unbalance caused GERD, and the close relativity between the unbalance of Ren-Du meridian and the dyskinesis of upper gastrointestinal has been proved. Recent study has proven that SCF/c-kit-ICC through Ca2+-CaM-MLCK pathways and PLC-Ca2+-PKC pathway regulating the stomach esophagus during contraction and relaxation of isolated smooth muscle function. So, we consider that this therapy could improve the unbalance of Ren-Du meridian, further influencing PLC-Ca2+-PKC and SCF/c-kit-ICC pacemaker and enhance Ca2+-CaM-MLCK pathways during contraction and relaxation of isolated smooth muscle of the function. In order to deeply study the essence about the meridian-molecular related pathogenesis, to further prove the scientific of the previous hypothesis, and also to provide the theoretical basis of the methods applying in other gastrointestinal dynamic disorders, we will investigate the main acupoints' skin temperature in Ren-Du meridian, PLC-Ca2+-PKC signaling pathways associated with the ICC and their relationship by BAFAT on GERD model rats.

英文关键词： Spleen and stomach;Beishu Acupoint Finger Acupuncture Therapy(BAFAT);Gastroesophageal reflux disease;Signaling pathways;Meridian skin temperature