Span衍生物的物理化学特性与木质纤维素高效酶解的关系

项目名称： Span衍生物的物理化学特性与木质纤维素高效酶解的关系

项目编号： No.21506216

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 有机化学

项目作者： 王闻

作者单位： 中国科学院广州能源研究所

项目金额： 21万元

中文摘要： 酶水解成本高是纤维乙醇技术实现产业化的主要瓶颈之一。添加非离子表面活性剂能提高酶解效率，减少酶用量，从而降低酶水解成本。申请人发现最适浓度的非离子表面活性剂能最大程度地提高木质纤维素酶解效率，然而对酶活力存在抑制。关于非离子表面活性剂促进木质纤维素高效酶解的机理尚未形成统一认识，从化学基团等微观尺度探讨非离子表面活性剂与木质纤维素高效酶解的关系的深入研究较少。本项目拟以非离子表面活性剂Span为起始物制备化学基团存在差异的系列衍生物，采用表面活性剂、酶学、界面化学和纤维乙醇等领域的分析技术，探讨Span衍生物的物理化学特性对纤维素酶、酶在经预处理的木质纤维素界面行为和纤维素可及性的影响，揭示具有高效酶解促进作用的Span衍生物类非离子表面活性剂的化学基团特性，为加深非离子表面活性剂与木质纤维素高效酶解关系的理论研究提供参考，为制备廉价高效的酶解促进剂提供理论指导。

中文关键词： 木质纤维素；纤维素酶；Span衍生物；酶水解

英文摘要： The high-cost of enzymatic hydrolysis is one of the main bottleneck problems which negatively influence the industrialization of cellulosic ethanol. The addition of nonionic surfactant in the enzymatic hydrolysis process can improve the hydrolytic efficiency, decrease enzyme amount, and then reduce the cost. However, the applicant found that the optimum concentration of nonionic surfactant which most enhanced the enzymatic hydrolysis of lignocellulose inhibited the enzyme activity. The mechanism on the improvement of nonionic surfactant to high efficiency enzymatic hydrolysis of lignocellulose has not been unanimous. Few studies, based on the chemical groups and structure, on the relationship between nonionic surfactant and high efficiency enzymatic hydrolysis of lignocellulose have been explored further. This item will use a nonionic surfactant – Span as the starting material to prepare series of derivatives which are different in chemical groups. The analytic technologies in the fields of surfactant, enzymology, interface chemistry and cellulose ethanol were adopted. The impacts of the physico-chemical properties of Span derivatives on the cellulase, the interface behavior of cellulase on the pretreated lignocellulose, and the accessibility of cellulase to cellulose were discussed deeply. The chemical groups properties of Span derivatives-liked nonionic surfactants which enhance the enzymatic digestion of lignocellulose will be expounded. This project will give impetus to the theoretic research on the relationship between the nonionic surfactants and high performance enzymatic hydrolysis of lignocellulose, and provide a theoretic instruction for preparing a more inexpensive and efficient promoter for enzymatic digestion of lignocellulose.

英文关键词： Lignocellulose;Cellulase;Span derivatives;Enzymatic hydrolysis