一类新颖结构的链霉菌源Vicenistations类抗肿瘤成分研究

项目名称： 一类新颖结构的链霉菌源Vicenistations类抗肿瘤成分研究

项目编号： No.31200262

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 杨胜祥

作者单位： 浙江农林大学

项目金额： 22万元

中文摘要： 放线菌是发现新颖活性天然产物重要来源，其中主要来源于链霉菌。Vicenistations是一类近年来从链霉菌里发现的具有显著抗肿瘤、抗菌活性的天然产物。 基于前期我们在vicenistations研究的基础上，我们又从大熊猫粪便里筛选到3株能产生vicenistations的链霉菌。以天然产物化学和现代生物发酵理论为基础，3株肿瘤细胞为靶标，在生物活性追踪指导下，对3 株链霉菌中新颖结构的vicenistations 类成份进行分离纯化，结构鉴定及抗肿瘤活性研究，有望从中筛选出理想的活性成分或先导化合物。除此之外，对产生目标化合物的菌株进行发酵条件优化，旨在为生产目标活性化合物提供有效途径。项目实施可为进一步为链霉菌来源的vicenistations类药物研究打下基础，对于创制出具有我国自主知识产权的抗肿瘤药物具有重要的理论价值和现实意义。

中文关键词： Vicenistations；生物活性；抗肿瘤；先导化合物；

英文摘要： A wide range of important secondary metabolites, including antibiotics, herbicides, and growth promoting substances, are produced by several members of the actinomycetes. Streptomyces is the largest antibiotic producing genus among the actinomycetes; more than 60% of the nearly 6000 antibiotics of microbial origin are produced by Streptomyces spp. including both antibacterials and antifungals and a considerable number of other bioactive compounds such as immunosuppressants and anticancer agents. Accordingly, Streptomyces strains, as prolific sources of a lager number and wider variety of new antibiotics, have been continuously noted rather than any other actinomycete genera. During the course of our investigation on the bioactive components of microorganisms, a new 20-atom macrocyclic lactam metabolite named sannastatin and two anoluges were isolated and identified from a culture broth of S. sannanensis, which was found in the feces of Ailuropoda melanoleuca.This is the first time that we discovered a vicenistatin analogues from this strain. Based on our previously research on vicenisations, we got another three Streptomyces strains which can produce vicenisations. Using three tumor cell as targets, Natural products chemistry and modern biofermentation theory as the basis, bioactivity-directed isolation was use

英文关键词： Vicenistations；Biological activity；Anticancer；Leading compound；