基于分子成像的非小细胞肺癌EGFR在体分子分型研究

项目名称： 基于分子成像的非小细胞肺癌EGFR在体分子分型研究

项目编号： No.81471724

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 孙夕林

作者单位： 哈尔滨医科大学

项目金额： 73万元

中文摘要： 以分子靶点表达差异为基础的分子分型是开展非小细胞肺癌（NSCLC）精准个体化诊疗的前提和重要依据，分子检验和分子病理能够实现离体下的分子分型，但在体分子分型方法却一直处于空白，亟待理论及技术上的突破。我们既往研究发现，借助18F标记的EGF及小分子化合物（MPG等）能够在体、实时、动态、定性及定量地揭示EGFR表达水平及突变状态。结合以往在EGFR靶向分子成像研究中取得的成果和形成的认识，本项目率先提出分子成像技术可实现NSCLC在体分子分型的假设，通过研发一系列EGFR过表达型、TKI敏感、耐药突变型靶向分子成像探针，开展基于MR/PET/CT分子成像的NSCLC在体分子分型新理论及新技术方法的研究，在离体及活体水平上评价及验证分子成像在体精准识别不同EGFR分子分型的价值，建立一种全新的基于分子成像在体分子分型策略，以期更精准地指导和开展NSCLC分子水平个体化诊疗，改善临床实践。

中文关键词： 分子影像学；分子分型；EGFR；非小细胞肺癌

英文摘要： Based on the differentially expressed molecular targets, molecular typing give very important information for non-small cell lung cancer (NSCLC) accurate personalized medicine. Molecular examination and molecular pathology provide the molecular typing information in vitro, but not in vivo. Molecular typing method in vivo has still been in the blank and needs theoretical and technological breakthroughs. Our previous studies have found that 18F labeled EGF or small molecule compounds (MPG, etc.) can real-time, dynamic, multivariate, qualitatively and quantitatively reveal EGFR expression and mutation status in vivo. Based on the past EGFR-targeted molecular imaging studies and experiences, this project first proposed the molecular imaging techniques can be used in vivo molecular typing NSCLC recognition hypothesis. By developing a series of EGFR over expression, mutant , second mutant targeted molecular imaging probes , we try to develop a MR / PET / CT molecular imaging new theories and method for molecular typing NSCLC in vivo . Simultaneously, evaluated our hypothesis in vitro and in vivo, and then establish of a new molecular imaging in vivo molecular typing strategy to carry out a more precise guidance and NSCLC individualized treatment at the molecular level, to improve clinical practice.

英文关键词： Molecular Imaging;Molecular Classification;EGFR;Non-small Cell Lung Carcinoma