长链非编码RNA TUG1调控牙周膜干细胞成骨分化及组织再生的研究

项目名称： 长链非编码RNA TUG1调控牙周膜干细胞成骨分化及组织再生的研究

项目编号： No.81470709

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 魏福兰

作者单位： 山东大学

项目金额： 73万元

中文摘要： 牙齿及牙周组织生物再生是临床亟待解决的难题之一。课题组前期将牙周膜干细胞用于牙齿及牙周组织再生，取得了良好的效果；同时，发现抑制miRNA-21可以促进牙周膜干细胞成骨分化及组织再生。长链非编码RNA（LncRNA）可以结合miRNA影响其负性调控作用，因此，我们筛选出与miRNA-21相关，在牙周膜干细胞成骨向分化中差异明显的LncRNA-TUG1。在此基础上，本课题进一步研究LncRNA-TUG1对牙周膜干细胞成骨分化的影响；将过表达或沉默LncRNA-TUG1的牙周膜干细胞，回植于小型猪牙周炎骨缺损区，研究LncRNA-TUG1对组织再生的影响；并探讨LncRNA-TUG1调控牙周膜干细胞成骨分化及组织再生的机制。通过本研究有望阐明牙周膜干细胞介导组织再生中lncRNA-TUG1的调控功能，为通过调控牙齿干细胞促进口腔组织再生提供进一步的理论和实验依据。

中文关键词： 牙周膜干细胞；组织再生；长链非编码RNA

英文摘要： Functional regeneration of tooth and periodontal tissue is a major challenge in clinic. Periodontal ligament stem cells (PDLSCs)-mediated regeneration of tooth and periodontal tissue has provided therapeutic strategies for the replacement of a diseased or damaged tooth. We previously found that microRNA 21(miRNA-21) plays a vital role in osteogenic differentiation and tissue regeneration of PDLSCs. Thus, we identified long non-coding RNA TUG1 (lncRNA-TUG1) related to miRNA-21 as a potential influential factor during the osteogenic differentiation of PDLSCs.In this project, we will use human PDLSCs as in vitro model and experimental swine periodontic bone defect as in vivo model to study the effect of lncRNA-TUG1 on the osteogenic differentiation of PDLSCs and tissue regeneration, and try to elucidate the mechanism by which lncRNA-TUG1 regulates the osteogenic differentiation and tissue regeneration. This study might demonstrate the regulatory mechanism of lncRNA-TUG1 during PDLSCs-mediated tissue regeneration and provide further evidence for stem cell-mediated tissue regeneration.

英文关键词： periodontal ligament stem cell;tissue regeneration;long noncoding RNA(LncRNA)