浓H3PO4联合H2O2预处理木质纤维素的机理及其对高基质浓度酶水解的调控

项目名称： 浓H3PO4联合H2O2预处理木质纤维素的机理及其对高基质浓度酶水解的调控

项目编号： No.21306120

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 化学工业

项目作者： 沈飞

作者单位： 四川农业大学

项目金额： 25万元

中文摘要： 以木质纤维素乙醇转化技术中高效预处理和高基质浓度酶水解两个关键环节为研究切入点，基于木质纤维素组分在浓磷酸中具有可溶性和再生性，且获得的再生纤维素具有低聚合度和结晶度等技术特征为基础依据，本研究提出浓磷酸联合H2O2预处理实际木质纤维素。深入探讨浓磷酸在预处理中与木质纤维素各组分的主要反应途径及解聚动力学；并探讨H2O2 在浓磷酸条件下的对木质纤维素的作用机理；以预处理机理研究结果为基础，评价该预处理方法对原料的适应性；结合高基质浓度酶水解，明确预处理对酶水解基质浓度提高的潜力和内在调控机制，并优化浓磷酸联合H2O2预处理工艺，改善高基质浓度酶水解效率和可发糖回收率。通过本项目的研究，可为木质纤维素原料提供一种绿色、条件温和、高效的预处理方式，并为纤维素乙醇生产提高生产效率，降低生产成本提供理论依据和技术参考。

中文关键词： 木质纤维素；浓磷酸联合过氧化氢；预处理；高浓水解发酵；预处理机制

英文摘要： In order to give better soulutions to the key isssues of high-efficient pretreatment and high-solids enzymatic hydrolysis in the conversion process of lignocellulose to bioethanol, a pretreament method for lignocellulose is being proposed using the concentrated H3PO4 with H2O2 according to the solubilitity of concentrated H3PO4 to lignocellulose,and regenerated cellulose with relatively low DP (degree of polymerization) and CS (crystallinity). In this wrok, the in-deepth investigations will be performed on the key chemical reaction paths of lignin, hemicellulose and cellulose during the dissolution process in concentrated H3PO4.The depolymerization kinetics of lignin, hemicellulose and cellulose will be deeply investigated as well. Moreover, the machensims of H2O2 in the concentrated H3PO4 pretreatment will also give a considerable investigation. Based on the machensims of concentrated H3PO4/H2O2 pretreatment, the adaptability of the pretreatment to different lignocellulosice materials will be evaluated. Meanwhile, the potential enhancement of substrate consistency for enzymatic hydrolysis will be seriously evaluated. The regulative mechanisms between the pretreatment conditions and high-solids enzymatic hydrolysis will also be investigated. Besides, the optimization of pretreatment for improving high-solids enz

英文关键词： Lignocellulose；Concentrated H3PO4 plus H2O2；Pretreatment；High consistency hydrolysis and fermentation；Reaction mechanisms