选择性化学发光分子开关阵列对多组分农残的可视化检测

项目名称： 选择性化学发光分子开关阵列对多组分农残的可视化检测

项目编号： No.21277145

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 环境科学、安全科学

项目作者： 关贵俭

作者单位： 中国科学院合肥物质科学研究院

项目金额： 80万元

中文摘要： 农药的广泛使用造成大量农药残留在环境及农产品中，已经给生态环境和人类健康造成严重威胁，国家亟需解决农药残留快速检测所面临的挑战。本研究项目针对多组分农残敏感检测的实际需求，重点克服化学发光自身选择性差的难题，拟在磁性探针表面构建选择性化学发光分子开关。（1）利用磁性粒子的类酶催化特性产生化学发光信号，并根据分子结合抑制表面自由基清除原理（见工作基础），发展化学发光增强型传感原理和方法；（2）通过对探针结构和表面识别基团的设计，阐明纳米探针对农药分子选择性识别与化学发光信号输出的相互关系，建立增强型化学发光分析方法，研究不同探针对农药分子的灵敏性、选择性和可靠性，发展对特定农药分子敏感的探针制备方法；（3）将不同的纳米探针组装成陈列芯片，结合成像分析，建立多组分农残定性定量分析新途径，实现痕量农药残留物实时、原位的可视化检测，为研发便携式、低成本农残检测传感器提供理论基础和技术支撑。

中文关键词： 磁性探针；化学发光；农残；可视化；

英文摘要： Wide use of pesticides has produced a mass of pesticide residues in environment and agricultural products, which have become a serious danger to ecological environment and human health. Thus there has been a pressing societal need to resolve the challenge in rapid detection of pesticide residues. In this proposal, aiming at the practical need for sensitive detection of multicomponent pesticide residues, our strategy is to develop a novel methodology for building chemiluminescent (CL) switches with intrinsic selectivity on magnetic nanoparticles, in order to overcome the lack of selectivity in traditional CL systems. (1) The CL signal will be produced due to the generation of superoxide on peroxidase-like magnetic nanoparticles, and the CL-enhanced sensing principle and method will be developed based on inhibiting the scavenging of surface radical by molecular binding. (2) We will design the structure and surface recognition molecules of nanoprobes and elucidate the relativity between selective binding of pesticide molecules and CL response signal. After studying sensitivity, selectivity and reliability of various nanoprobes, we will propose the strategies for synthesizing CL nanoprobes with sensitivity to given pesticide molecules. (3) The nanoprobes with different sensitivity will further be assembled into micr

英文关键词： magnetic probes；chemiluminescence；pesticide residues；visual detection；