胃癌患者苔色变化的微生态-炎症免疫-代谢机制研究

项目名称： 胃癌患者苔色变化的微生态-炎症免疫-代谢机制研究

项目编号： No.81473593

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 詹瑧

作者单位： 南京中医药大学

项目金额： 73万元

中文摘要： 中医认为，白苔主表证、寒证，黄苔主里证、热证，灰黑苔多主热证。现代研究认为寒证、热证多与机体炎症免疫状态相关，而微生态失调是炎症免疫发生的重要因素，总结既往研究工作，提出工作假说：苔色与舌苔微生态菌群结构相关，是机体炎症免疫和代谢状态的外在映像。本项目拟开展基于医院的病例-对照研究，原发性胃癌100例，体检无重大器质性病变的体检者120例为对照，要求性别、年龄匹配。基于微生物系统分类学的理想指标-16S/18S rRNA基因联合高通量测序，解析舌苔微生态菌群结构；基于代谢组学分析血清小分子代谢产物，鉴定胃癌患者苔色相关标志代谢分子；基于标志代谢分子网络数据库模拟炎症免疫、能量代谢等信号通路，免疫检测血清10-20个炎症分子验证机体炎症免疫途径。通过本项目研究，揭示胃癌发生、苔色变化的微生物-炎症免疫-代谢机制，阐明胃癌患者苔色变化的生物学原理，为胃癌证候规范化研究提供科学依据。

中文关键词： 胃癌；苔色；微生态；炎症免疫；代谢组学

英文摘要： The concept of traditional Chinese medicine (TCM) tongue diagnosis holds that white coating represents the exterior syndrome and cold syndrome, yellow coating represents interior syndrome and hot syndrome, and gray and black coating always represent hot syndrome. Modern researches have proved that both cold and hot syndromes are closely related to the status of inflammation which plays an important role in the process of microflora imbalance. Based on the privious studies, this project propounds the working hypotheses that the coating-color hand in hand with the tongue-coating microflora and is the exterior image of the body's metabolism. To test the working hypotheses, the project will conduct a hospital-based case-control study with a population with 100 patients with primary gastric cancer and 120 people who have the medical exam with no severe organic wrong as control. The tongue-coating microflora is investigated by the next-generation sequencing technologies in 16S/18S rRNA genes. The serum metabonomic biomarkers of coating colors are identified by metabonomics studies on GC×GC-TOF MS. Different biomarkers associated with gene signaling pathways, such as inflammation and energy metabolism, are analyzed by network database. The inflammation signaling pathways are confirmed by quantitative analysis of 10 to 20 serum inflammation associated moleculars. The aim of this project is to draw out the assocication among gastric cancer risk, the change of coating colors and the tongue-coating microflora, serum inflammation, serum metabonomics in the patients with gastric cancer. The findings will attempts a definition of biological cause of coating color and give the scientific basis for the differentiation of TCM Zheng in gastric cancer.

英文关键词： Gastric cancer;Coating color;Microflora;Inflammation and immunity;Metabonomics