脉冲电磁激励骨髓间充质干细胞移植修复脊髓损伤的实验研究

项目名称： 脉冲电磁激励骨髓间充质干细胞移植修复脊髓损伤的实验研究

项目编号： No.81501889

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 李玉琳

作者单位： 天津医科大学

项目金额： 18万元

中文摘要： 脊髓损伤（SCI）发病率高、致残率高，细胞移植治疗是目前的研究热点。如何获得高活性的“种子细胞”是急需解决的问题。课题组发现，单纯移植骨髓间充质干细胞（BMSCs）修复脊髓损伤的疗效有限，主要因其在抑制性微环境的存活、增殖及分化能力较低。而经脉冲电磁场（PEMF）激励后的BMSCs其活性及增殖分化能力可以得到显著增强。但最佳的激励强度、潜在的分子机制和移植修复脊髓损伤的疗效仍不清楚。本课题拟采用不同强度的PEMF激励BMSCs，以选择最佳的干预强度；将最佳强度PEMF激励后的高活性BMSCs移植修复SCI，观察其在大鼠体内增殖分化情况和大鼠运动功能恢复水平；进一步采用Western-blot技术检测Notch信号通路相关受体及配体表达水平与PEMF提高BMSCs活性的相关性，初步阐述其潜在的分子机制和信号通路。为脊髓损伤的修复提供新的思路。

中文关键词： 脊髓损伤；脉冲电磁场；骨髓间充质干细胞；细胞移植

英文摘要： Spinal cord injury (SCI) is a disease with a high incidence rate and high disability rate. Cell transplantation therapy is the hot spot of current research for treating spinal cord injury. How to obtain the high activity of the seed cells is an urgent problem to be solved. Our team found that the treatment effect of transplantation of bone marrow mesenchymal stem cells (bone mesenchymal stem cells, BMSCs) was limited, mainly due to its low ability in the survival, proliferation and differentiation of the suppressant microenvironment. After excited by pulsed electromagnetic fields (PEMF), BMSCs’ activity, proliferation and differentiation ability can be significantly enhanced. However, the best intensity of the excitation, the potential molecular mechanisms and the effect of transplantation in treating spinal cord injury are still unclear. In this topic, we will use different intensities of PEMF to excite BMSCs and select the best intervention intensity; We will use the selected BMSCs to repair SCI in rats and observe its proliferation and differentiation and the rats motor function; Further, we will use western-blot technology to detect the level of Notch signaling pathway related receptor and ligand expression, and BMSCs activity and PEMF, initially on its potential molecular mechanism and signal pathway. Overall, we want to provide a new way for repairing spinal cord injury.

英文关键词： spinal cord injury;pulsed electromagnetic field;bone mesenchymal stem cell;cell transplatation