精确控制蛋白质与材料表界面的相互作用及其作用机制研究

项目名称： 精确控制蛋白质与材料表界面的相互作用及其作用机制研究

项目编号： No.21334004

项目类型： 重点项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 陈红

作者单位： 苏州大学

项目金额： 310万元

中文摘要： 生物材料首先通过其表面与生物体发生相互作用，进而诱导特异性的生物响应过程，最终实现材料的生物学功能。而蛋白质作为生命活动的主要调控者，其在材料-生物界面中的行为，尤其是与生物材料表面的相互作用不仅是生物表界面研究中的核心问题，而且也是生物技术应用领域中的关键问题。本项目为了克服蛋白质性质的多变量差异对揭示研究规律造成的困难，运用分子克隆、基因突变和重组等分子生物学技术获得一系列单点突变或分子量、等电点单一理化性质渐变及生物活性可调的模型蛋白质体系，并根据该蛋白质体系的结构特点，对典型材料表面的化学结构进行调控；通过研究材料表面性能与蛋白质理化性质之间的对应关系，阐明蛋白质与材料相互作用过程中的关键影响因素，建立材料表面性质与蛋白质理化性质间的相互作用关系，揭示材料表面调控蛋白质结合与活性的规律，为蛋白质的检测，设计与制备生物相容性、功能性材料提供理论依据和实验支撑。

中文关键词： 蛋白质;材料表界面;精确控制;相互作用;

英文摘要： Understanding protein-surface interactions is one of the most important aspects in the development of biomaterials. The reason for the great interest in this aspect is the realization that the behavior of proteins at the surface of a biomaterial is fundamentally responsible for the biocompatibility, the biofunctionality and the ultimate performance of the material. However, the complexity of protein structures as well as the sheer abundance of different types of proteins in physiological fluids renders this problem very complex and challenging for researchers. This project seeks to overcome such challenges by developing systematically model proteins with controlled and tunable properties using modern molecular biotechniques such as gene mutation, gene recombination and molecular cloning. This strategy, in combination with our experience and continued development in advanced surface modification/functionalization techniques and sophisticated characterization methodologies, will enable us to acquire protein-surface interactions at the fundamental level. This project aims at revealing the critical parameters in governing protein-surface interactions and establishing basic principles underlying surface properties and protein behavior, through a thorough examination of the correspondence relationship between surface properties of typical biomaterials and the physicochemical properties of proteins. The outcome of this project is expected to provide important theoretical basis and experimental support for the rational design and development of biomaterials, biomedical devices as well as novel biotechniques that involve protein-surface interactions.

英文关键词： protein ;material surface;precise control;interaction