水稻OsLG1基因调控穗型的分子机理

项目名称： 水稻OsLG1基因调控穗型的分子机理

项目编号： No.31471457

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 朱作峰

作者单位： 中国农业大学

项目金额： 100万元

中文摘要： 穗型是影响水稻产量的重要农艺性状，也是水稻驯化和遗传改良的重要目标。解析穗型调控与进化的分子机理是水稻遗传与进化研究领域重要课题之一。在国家自然科学基金的资助下，我们克隆了控制野生稻散穗的OsLG1基因，发现在水稻驯化过程中，选择该基因上游11 kb附近的顺式调控元件变异导致栽培稻穗型变紧。本项目拟在此基础上，鉴定功能性变异区域内的一个长链非编码RNA对OsLG1基因的调控作用；同时，采用正向与反向遗传学的方法，分离、鉴定OsLG1基因调控的靶基因及控制穗型的新基因，进一步解析OsLG1基因调控水稻穗型的分子机理。研究结果不仅为解析水稻穗型调控和进化的分子机理这一重要科学问题提供证据，同时还为穗型的分子改良提供重要参考。

中文关键词： 水稻；OsLG1；穗型；分子机制

英文摘要： Panicle architecture is a key agronomical factor determining grain yield and thus has been a major target of rice domestication and improvement. Revealing the molecular mechanism of the regulating and domestication of the panicle architecture is one of the key research subjects in the research area of rice genetics and domestication. Funded by NSFC, we have cloned the OsLG1 gene controlling the panicle transition during rice domestication using map-based cloning method. The regulatory region mutations residing in about 11 kb upstream of the OsLG1 gene, result in a compact panicle architecture in cultivars during rice domestication. In this project, we will further identify the role of a long non-coding RNA lying in the 11 kb upstream region, and reveal the molecular mechanism of regulating OsLG1 gene. Meanwhile, we will identify the target gene regulated by OsLG1 and new genes controlling panicle architecture using forward genetic and reverse genetic methods. We will further reveal the molecular genetics mechanism of the OsLG1 gene regulating rice panicle architecture. The results will provide molecular evidence for understanding molecular mechanism of domestication and regulating of rice panicle architecture, and provide theory for improving rice panicle architecture using molecular breeding method.

英文关键词： rice;OsLG1;panicle architecture;molecular mechanism