基于纳米纤维构筑的量子点-荧光素荧光共振能量转移体系的结构与性能

项目名称： 基于纳米纤维构筑的量子点-荧光素荧光共振能量转移体系的结构与性能

项目编号： No.51273157

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 董丽杰

作者单位： 武汉理工大学

项目金额： 80万元

中文摘要： 以荧光素（OFM）为FRET受体、以能量匹配的超稳定荧光量子点(QDots)为FRET供体，采用静电纺丝技术，制备一类QDots高度有序排列的聚合物基复合纳米纤维。以纳米纤维为模板，将OFM组装在聚合物纳米纤维的表面，调控Qdots的结构与排列、控制纳米纤维的直径以及荧光素的负载密度，可以有效调节QDots-OFM的F?rster间距在1-10 nm，从而构筑一类基于纳米纤维的具有高FRET效率的新型QDots-OFM FRET体系。这一研究不仅通过加工诱导构筑了功能基元有序排列的新的超分子结构，同时，通过调节纳米纤维的直径尺寸，有效调整QDots与临近iOFM的F?rster间距，从而提高FRET体系的双荧光信号响应性能。对深入研究材料-结构构筑-加工调控-性质优化的相互作用规律，研制一类具有独立知识产权的高效、灵敏、精确的比率型荧光纳米探针具有重要的理论研究意义和应用价值。

中文关键词： 纳米纤维；量子点；荧光共振能量转移体系；静电纺丝；荧光探针

英文摘要： Fluorescence resonance energy transfer （FRET）occurs when the electronic excitation energy of a donor chromophore is transferred to an acceptor molecule nearby via a dipole-dipole interaction between the donor-acceptor pair. The FRET process is more ef?cient when there is an appreciable overlap between the emission spectrum of the donor and the absorption spectrum of the acceptor. The strong distance-dependence of the FRET ef?ciency has been widely utilized in studying the structure and dynamics of proteins and nucleic acids, in the detection and visualization of intermolecular association and in the development of intermolecular binding assays. In this work, we would like to develop a facile and efficient approach towards FRET system which donor-acceptor complexes will be formed based on polymer-based nanofibers. Modified quantum dots (QDots) by long-chains are controlled align into 10-20 nm polymer-based nanofibers via electrostatic spinning technology, while collective fluorescein are attached to the surface of polymer nanofibers with QDots. The restricted distance between QDots and fluoresceins nearby bring the quantum dots and fluorescein together close enough to allow the resonance dipole-dipole coupling required for ?uorescence resonance energy transfer (FRET) to occur. Due to their giant FRET effect, th

英文关键词： Nanofiber；Quantum Qdots；FRET；Electrospinning；Fluorescence probe