鞍带石斑鱼仔稚鱼对糖的利用能力及代谢调控机制研究

项目名称： 鞍带石斑鱼仔稚鱼对糖的利用能力及代谢调控机制研究

项目编号： No.31260641

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 吴小易

作者单位： 海南大学

项目金额： 50万元

中文摘要： 本项目以鞍带石斑鱼仔稚鱼为研究对象，采用仔稚鱼培育、生化分析、组织学观察、RT-PCR、Realtime-PCR和数理统计等综合研究方法，从糖在鱼体内的消化、转运及代谢入手，重点研究仔稚鱼摄食糖后生长性能的变化、胃肠道结构的变化、消化系统对糖的消化分解能力（糖消化酶活性）、血液葡萄糖水平的变化、肝脏和肌肉组织细胞葡萄糖转运能力（葡萄糖转运蛋白GLUTs基因表达类型及表达水平）及糖代谢（糖酵解、糖异生、磷酸戊糖途径、柠檬酸循环、糖原合成、糖原分解）能力（代谢关键酶活性），鉴定其肌肉和肝脏组织中GLUTs类型，探索血糖水平与消化系统糖消化酶活性、肝脏和肌肉组织细胞GLUTs基因表达水平及糖代谢关键酶活性之间的关联，阐明鞍带石斑鱼仔稚鱼糖消化、转运及利用能力，揭示其糖代谢部分调控机制，为开发鞍带石斑鱼仔稚鱼高效糖利用技术奠定基础。项目的实施可丰富海水鱼类仔稚鱼营养学理论，会有一定的科学意义。

中文关键词： 鞍带石斑鱼仔稚鱼；糖利用；糖代谢；糖消化；糖转运

英文摘要： This project aims to study carbohydrate utilization and glycometabolic regulations' mechanism in grouper Epinephelus lanceolatus frys. Growth performance, stomach and intestine structure, digestive ability of carbohydrate, blood glucose levels, glucose transport capcity of liver and muscle tissue cells for carbohydrates fed Epinephelus lanceolatus frys will be focused on. Research methods such as fry culture, biochemical analysis, histological observation, RT-PCR, Realtime-PCR, mathematics statistics and so on, will be applied in this project. Carbohydrate digestive enzymes activities in the degestive system in carbohydrates fed frys will be measured, and also, activities of key enzymes in glycometabolism, including glycolysis, gluconeogenesis,pentose phosphate pathway,tricarboxylic acid cycle, glycogenolysis and glycogenesis, will be analyzed.These key enzymes contain glucokinase, 6－phosphofructokinase, pyruvate kinase, glucose-6-phosphatase, pyruvate carboxylase, phosphoenolpyruvate carboxykinase, Fructose-1，6-diphosphatase, glycogen synthase, glycogen phosphorylase, glucose-6-phosphate dehydrogenase and citrate synthase. Gene express patterns and levels of glucose transporters (GLUTs) in liver and muscle tissue cells will be identified or measured by using molecular tools. Through the research works above, ki

英文关键词： Epinephelus lanceolatus larvae；Carbohydrate utilization；Carbohydrate metabolism；Carbohydrate digestion；Carbohydrate transportation