基于激发态分子内质子转移调控的接力识别荧光探针研究

项目名称： 基于激发态分子内质子转移调控的接力识别荧光探针研究

项目编号： No.21476029

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 有机化学

项目作者： 汤立军

作者单位： 渤海大学

项目金额： 85万元

中文摘要： 接力识别是荧光探针分子功能强化的新方向。本项目旨在通过对荧光探针的激发态分子内质子转移（ESIPT）过程的调控实现重要金属离子和阴离子的接力识别。拟以典型的具有ESIPT性质的荧光团为基本骨架，设计合成三类含不同共轭体系和取代基团的荧光探针。利用探针分子中ESIPT质子供体基团和离子识别基团的协同作用实现金属离子的识别，原位生成的探针-金属离子配合物通过金属离子取代实现阴离子识别；通过ESIPT的质子供体基团与金属离子的先结合后释放控制ESIPT状态，从而实现基于ESIPT调控的接力识别功能。考察不同取代基、共轭体系对探针分子的ESIPT性质及接力识别功能的影响，探索结构与性能间相关规律，力求获得系列具有长波长发射、比率荧光响应的接力识别功能荧光探针，为基于ESIPT调控的接力识别荧光探针的设计合成提供理论依据。相关结果在接力识别功能荧光探针研究方面具有广阔的应用前景和重要的科学意义。

中文关键词： 荧光探针；接力识别；激发态；质子转移；调控

英文摘要： Relay recognition is a new research direction for function intensify of fluorescent probes. The objective of this project is to realize relay recognition of important metal ions and anions through modulating the excited-state intramolecular proton transfer (ESIPT) process of fluorescent probes. Based on typical ESIPT featured fluorophores such as benzoxazole, benzothiozole, benzoimidazole and 1,3,4-oxadiazole derivatives,we plan to synthesize three classes of fluorescent probes that contains different conjugative structure and substituents. By utilizing the synergistic interaction of ESIPT proton donor and receptor moiety of the the probe with a specific metal ion to realize certain metal ion recognition, the in situ generated probe-metal ion complex is used as an indirect probe for anion recognition via metal ion displacement approach. The ESIPT status of the probe is controled by metal ion binding and releasing processes, thus realize the relay recognition function of the probes through modulating ESIPT. Moreover, it is also necessary to clarify the effect of probe structure on ESIPT property and the structure-activity relationship between probe structure and relay recognition behavior through investigating the effects of substituents and conjugation systems on the ESIPT and relay recognition behaviors of the synthesized probes. Through exploration of the structur-property relationships, it is aimed to obtain a series of realy recognition function fluorescent probes that with long wavelength emission and ratiometric emission reponses to target ion species. These investigations will provide theoretical foundation for the design and synthesis of relay recognition fluorescent probes based on modulating ESIPT. The related results have broad applicability and significant scientific meaning in the research fields of relay recognition function fluorescent probes.

英文关键词： Fluorescent probe;Relay recognition;Excited-state;Proton transfer;Modulation