芍药甘草"酸甘化阴"调节Glu-GABA平衡的"柔筋止痉"中枢作用机制研究

项目名称： 芍药甘草"酸甘化阴"调节Glu-GABA平衡的"柔筋止痉"中枢作用机制研究

项目编号： No.81202934

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 王景霞

作者单位： 北京中医药大学

项目金额： 23万元

中文摘要： 芍药甘草汤是《伤寒论》的名方，为酸甘化阴，柔筋止痉的代表方剂，其配伍精当，治疗痉挛性瘫痪疗效确切。课题组在对其配伍现代研究的基础上，结合中西医对脑卒中后痉挛性瘫痪的认识，以中医学"阴阳平衡"理论为指导，采用脑卒中痉挛偏瘫动物模型，观察芍药甘草不同比例的止痉作用，研究两药配伍之后的协同增效作用和最佳剂量配比；并通过观察两药配伍对脑内抑制性神经递质和兴奋性神经递质的含量及其受体的影响，揭示脑卒中痉挛状态"GABA-Glu"系统的病理变化，探讨其酸甘化阴、柔筋止痉的作用特点；进一步采用NMDA兴奋性神经毒细胞模型，借用NMDA受体、GABA受体激动剂和拮抗剂，研究"Src-NR2-nNOS"信号介导的受体之间的相互作用，探讨芍甘配伍调控"GABA-Glu"平衡的分子机制。本课题旨在阐明芍药甘草"酸甘化阴"的配伍特色和优势，揭示中药"柔筋止痉"的现代科学内涵，是中药五味理论的有益探索。

中文关键词： 芍药甘草汤；酸甘化阴；阴阳平衡；痉挛；神经递质

英文摘要： Shaoyao Gancao Tang is a famous formula in Shang Han Lun. It has the functions of norishing blood and yin,pacifying liver,and relieving spasm and pain. It is representative of formulas that has the actions of sour and sweet tranforming into yin and relaxing muscles and relieving spasm. This compatibility is simple and delicate. And its curative effect on spastic paralysis is accurate. On the basis of modern researches of this compatibility combining with knowledge of poststroke spasticity in Chinese and western and guided by "yin-yang balance" theory,we plan to observe the effect of different dose compatibility of Shaoyao and Gancao on neuroethology assessment and pain threshold of post-stroke spasticity rats in order to get the optimum dose and synergism. Then to detect inhibitory and excitatory neurotransmitters content and relevant receptors expression in brain of post-stroke spasticity rats to reveal the cerebral pathological changes and investigate characteristics of the compatibility. Then, we plan to observe the interaction of GABA and NMDA receptors in NMDA-induced excitoxicity cortex cells and neuroprotection by blood serum or cerebrospinal fluid containing Shaoyao and Gancao. We also detect "Src-NR2-nNOS" signal targets comparing with receptor agonist and antagonist. This project will explore the rela

英文关键词： Shaoyao Gancao Tang；Sour and sweet tranforming into yin；Yin-yang balance；Spasticity；Neurotransmitters