靶向降冰片烯共聚物在癌细胞荧光成像中的应用

项目名称： 靶向降冰片烯共聚物在癌细胞荧光成像中的应用

项目编号： No.21204052

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 高分子科学

项目作者： 谢楠

作者单位： 首都医科大学

项目金额： 25万元

中文摘要： 癌症是当今威胁人类健康和生命最严重的疾病之一，早期发现并及时治疗是战胜癌症的首要问题。传统的癌症 检测方式受到体内代谢产物浓度太低的限制，很多特异性指标检测精度不高；基于微观细胞层面的荧光成像技术，能够实现对癌变组织或细胞的可视化灵敏检测，有可能大大提高早期癌症特异性指标的检测精度，但也对探针材料提出了水溶、靶向等复合功能化的需求。本项目拟通过化学修饰的方法，分别设计合成水溶性、靶向作用、荧光示踪的降冰片烯单体，采用Grubbs催化剂通过开环易位聚合的方式直接得到具有复合功能的高分子材料，利用荧光基团实现多谱段/信道下的光学影像示踪，利用靶向片段识别肝癌、肺癌、宫颈癌细胞，甚至是不同细胞器，实现特异性的荧光成像，在细胞层面上实现对癌变的识别，发展具有实际应用前景的新型多功能生物相容高分子材料。

中文关键词： 荧光成像；靶向；降冰片烯共聚物；开环易位聚合；水溶

英文摘要： Cancer, or malignant tumor, is one of the most serious threatens for human health and life. It is widely accepted that successful cure of most cancers primarily relies on early detection and timely treatment. Clinical diagnosis for early-stage cancer mainly depends on the definition of specific products of metabolism or tissue slides, which remains at the macrofunctional and anatomical level and limited to many factors. Molecular imaging (such as fluorescence imaging, etc.) techniques are now indispensable tools in modern diagnostics, because they are highly specific, enable visualization of some disease processes and provide biological information at the molecular level in living systems. To support the unmet needs for molecular imaging, there has been considerable interest in investigating highly specific targeted and labelled imaging probes. Therefore, this proposal intends to design and synthesize functionalized norbornenes with capabilties of water-solubility, targeting, fluorescent labelling through chemical modification, prepare polymeric imaging probes directly via a random ring-opening metathesis polymerization (ROMP) using Grubbs' catalyst as an initiator, and realize optical fluorescence imaging for diverse tumor cells or specific organelles on the cellular level through targeting function groups and

英文关键词： Fluorescence imaging；Targeting；Norbornenyl copolymer；ROMP；Water-soluble