猪链球2型双组份调控系统1910HK/RR调控机制研究

项目名称： 猪链球2型双组份调控系统1910HK/RR调控机制研究

项目编号： No.31302123

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 袁芳艳

作者单位： 湖北省农业科学院

项目金额： 25万元

中文摘要： 猪2型链球菌病是由猪链球菌2型（SS2）引起的一种严重危害人畜健康的传染病。SS2致病机理研究是当前热点和难点。双组份调控系统（TCS）是致病菌中普遍存在的一种跨膜信号转导机制，在细菌代谢、调节毒力基因表达等方面发挥着重要作用。课题组前期研究结果发现，SS2国内流行株SC19双组份调控系统1910rr/hk 缺失后毒力显著降低，但作用机理尚不清楚。本项目拟进行研究（1）运用基因表达谱芯片筛选差异表达基因并通过荧光定量PCR验证；（2）通过染色质免疫共沉淀测序及凝胶迁移率试验鉴定1910rr/hk直接调控基因及DNA 序列；（3）利用基因缺失或过表达等方法研究受调控的部分功能基因。本项目旨在绘制SS2中1910rr/hk基因调控网络模型，阐明其调控机理，为SS2致病机理研究打下基础。

中文关键词： 猪链球菌2型；双组份调控系统1910HK/RR；调控；差异表达基因；毒力和致病性

英文摘要： Streptococcus suis serotype 2 (SS2) is considered an important zoonotic disease pathogen which causing great economic loss to the pig industry worldwide. In recent years, research focused on the pathogenesis and prevention has been a hot spot. Bacterial two-component regulatory system (TCS) plays an important role in bacterium metabolism and regulation of virulence gene expression. Early study by our group demonstrated that deletion of the TCS 1910HK/RR in SS2 significantly reduced the virulence of SS2. However, the mechanisms still need further elucidation. This study intends to (1) select and validate differential expression genes between wild type strain and 1910rr/1911hk deletion strain by DNA microarray and real-time quantitative RT-PCR; (2) identify the genes and binding sequence of 1910HK/RR TCS regulation by ChIP-seq and electrophoretic mobility shift assay (EMSA); (3) further research some gene function of 1910 HK/RR TCS regulation by gene deletion or overexpression methods. Our aim is to systemically clarify how 1910rr/hk TCS regulates gene expression and further contributes to the virulence at the molecular level. The research would lay foundation for the development of prevention and control technology against SS2 and provide new sights of its pathogenesis.

英文关键词： Streptococcus suis serotype 2；Two-component regulatory system；1910HK/RR；regulation；virulence and Pathogenicity