稀土纳米模拟酶的制备及其在生物传感中的应用研究

项目名称： 稀土纳米模拟酶的制备及其在生物传感中的应用研究

项目编号： No.21475001

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 王伦

作者单位： 安徽师范大学

项目金额： 84万元

中文摘要： 本项目拟采用溶剂热法，控制合成和修饰条件，制备具有不同组成、形貌、粒径、表面修饰、价态、电荷等系列稀土掺杂纳米材料（包括空心结构，核壳结构，立方结构，纳米球，纳米棒等），用作纳米模拟酶。以电化学发光分析方法作为检测手段，系统地研究稀土纳米模拟酶催化性能与其结构、形态之间的关系，调控稀土纳米材料模拟酶催化活性，提高它们的稳定性、特异性和催化效率，并从理论上给予解释。同时，通过选择高效的共反应剂，或者采用功能材料修饰电化学发光体系，改进提高电化学发光性能。建立基于稀土纳米模拟酶的电化学发光分析体系，应用于生物传感，实现抗原、抗体、疾病标志物、食品和环境中有害物等物质的快速、准确、廉价、高灵敏、高选择性的检测，以期为疾病诊断、食品和环境中有害物的分析检测等领域提供新材料、新技术、新方法。

中文关键词： 纳米材料；生物传感器；电分析化学；生物分析化学

英文摘要： The project intends to prepare series of rare-earth doped nanomaterials with different composition, morphology, particle size, surface modification, valence, charge, etc (including the hollow structure, core-shell structure, cubic structure, nano ball, nanorods, etc.), through solvothermal technology and controlling conditions of synthesis and modification. These nanometer materials will be used as nano-mimic enzymes. By the means of electrochemiluminescence analysis method, the relationship between the catalysis performance of rare-earth nano enzymes mimic and its structure and morphology will be studied systematically. To improve their stability, specificity and catalytic efficiency, we would regulate the catalytic activity of rare-earth nano-mimic enzymes and explain the phenomenon theoretically. Meanwhile, by selecting the efficient reaction agent, or using functional materials to modify electrochemiluminescence system, to improve the electrochemiluminescence performance. The final purpose is to develop electrochemiluminescence systems based on the rare-earth nano-mimic enzymes, applying to biological analysis and realizing rapid, accurate, cheap, high sensitive, high selective detection of the antigen, antibody, disease markers, harmful substances in food and environment, which would provide new materials, new technology and new method in the areas of disease diagnosis, detection of pests in food and environment.

英文关键词： nanomaterial;biosensor;electroanalytical chemistry;bioanalytical chemistry