This volume contains the proceedings of ICE'23, the 16th Interaction and Concurrency Experience, which was held at the NOVA University in Lisbon, Portugal, as a satellite event of DisCoTec'22. The ICE workshop series features a distinguishing review and selection procedure: PC members are encouraged to interact, anonymously, with authors. The 2023 edition of ICE included double blind reviewing of original research papers, in order to increase fairness and avoid bias in reviewing. Each paper was reviewed by three PC members, and altogether 5 papers were accepted for publication plus 4 oral presentations which are not part of this volume. We were proud to host 2 invited talks, by Carla Ferreira and Adrian Francalanza. The abstracts of these talks are included in this volume, together with the final versions of the research papers, which take into account the discussion at the workshop and during the review process.
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