单相抑郁症与5-HTR信号转导通路基因-环境作用机制研究

项目名称： 单相抑郁症与5-HTR信号转导通路基因-环境作用机制研究

项目编号： No.31271093

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 医药、卫生

项目作者： 杨艳杰

作者单位： 哈尔滨医科大学

项目金额： 80万元

中文摘要： 抑郁症(Depression)以高发病率、高复发率引起广泛关注，WHO预计2020年抑郁症将成为人类的第二大杀手，而其发病机制至今尚未清楚。5-HT系统功能紊乱是抑郁症遗传易感性的主要假说，但目前涉及5-HT受体及其信号转导通路相关候选基因5-HT1A、5-HT2A、5-HT1B受体与G蛋白的GNβ3、CREB的基因多态性及基因-环境交互作用与抑郁症的关系尚未见到。本研究在知情同意的原则下，采用病例对照的研究方法，在哈尔滨医科大学附属第一医院收集单相抑郁症患者和对照者各600 例，采集外周静脉血并调查相关危险因素；单碱基延伸SNP分型技术（SNaPshot）方法检测基因多态性；通过多因子降维法、logistic 回归等方法分析基因-基因、基因-环境之间交互作用与单相抑郁症的关系，探索遗传易感因素和环境因素对单相抑郁症的影响，阐明单相抑郁症的发病机制，为抑郁症的预防及早期诊治提供依

中文关键词： 抑郁症；5-HT信号转导通路；单核苷酸多态性；环境因素；交互作用

英文摘要： Depression has caused widespread concern because of the high incidence and high recurrence rate, the WHO expected that in 2020, the depression will become the second dangerous killer of the human, and its pathogenesis is still not clear. 5-HT system dysfunction is the main hypothesis of genetic predisposition to depression. But there was rarely research involved 5-HT receptor signal transduction pathway candidate gene such as 5-HT1A receptor gene, 5-HT2A receptor gene, G-protein GNβ3 gene and CREB gene polymorphism. And the relationship between the depression and gene-enviroment interaction has not yet comfirmed currently. In the study, with the principle of informed consent, the case-control study is commited, and 600 unipolar depression patients of the First Affiliated Hospital of Harbin Medical University and 600 healthy persons were invited in this study. We collected the peripheral blood samples to investigate the related risk factors of depression. Single base extension SNP genotyping technology(SNaPshot) is commited to detect the genetic polymorphism. At the same time, the drop through a multipel-factor-dimensional method and logistic regression analysis is performed to analyze the relationship of gene-gene and gene-enviroment interaction, and explore the affection of genetic susceptibility and enviroment

英文关键词： MDD；5-HT system signal transduction pathway；single nucleotide polymorphism；environmental factors；interaction