羧甲基壳聚糖自支撑体系构建与组装机理研究

项目名称： 羧甲基壳聚糖自支撑体系构建与组装机理研究

项目编号： No.U1504527

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 一般工业技术

项目作者： 刘文涛

作者单位： 郑州大学

项目金额： 27万元

中文摘要： 靶向药物对防治我国高发性乳腺癌疾病具有重要意义。超分子组装结构作为靶向药物的载体可以增强药物的疗效，减小副作用。组装体结构对于药物负载和释放具有决定性的影响，但形成机制不详。由此导致不能有效控制自组装结构的重复性和均匀性，药物的控缓释行为也难以预测，制约了靶向技术的临床应用和推广。我们前期对壳聚糖自组装结构的研究发现和证实了壳聚糖的线性组装机制，考察了多孔二氧化硅-壳聚糖药物载体对模型药物的包封率和缓释行为。本课题拟利用分子自组装和仿生合成方法制备负载模型药物三羟基异黄酮、具有“遥爪”结构的羧甲基壳聚糖-碳酸钙自支撑体系，应用QCM、AFM等技术实时监测结构变化，微观力学谱测试力学性能，揭示自支撑体系中非健力的相互作用机理和可能的微观结构。该项研究将为乳腺癌的防治提供新思路和新材料。

中文关键词： 羧甲基壳聚糖；自支撑；组装；乳腺癌肿瘤细胞；靶向

英文摘要： Targeted drug is of great significance to prevent and treatment high incidence of breast cancer disease in China. As targeted drug carriers, supramolecular assembly structure may enhance the efficacy of the drug and reduce the side effects. Assembly structure for drug loading and release have a decisive influence, but the mechanism is unknown. Resulting in not effectively control in the repeatability and uniformity of self-assembled structures, and controlled release drug behavior is difficult to predict, which restricts clinical application and promotion of the targeted technology. Our previous study on the self-assembly of chitosan found and confirmed linear assembly mechanism. The effects of porous silica - chitosan drug carrier on model drug encapsulation efficiency and sustained release behavior were investigated, too. This project proposed a model drug freestanding system loaded genistein, with carboxymethyl chitosan-calcium telechelic structure by molecular self-assembly and biomimetic synthesis method. QCM, AFM will be used to monitor the real-time structural changes, and the domain mechanical properties will be revealed with micro-mechanics mapping. The results will reveal a freestanding system interaction mechanism of weak interaction and possible microscopic structures. This study will provide new ideas and new materials for breast cancer prevention.

英文关键词： Carboxymethyl chitosan;Freestanding;Assembly;Breast cancer cells;Targeted