等离子自组装纳米超结构对神经细胞分化实时动态分析

项目名称： 等离子自组装纳米超结构对神经细胞分化实时动态分析

项目编号： No.21301073

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 徐丽广

作者单位： 江南大学

项目金额： 30万元

中文摘要： 等离子纳米超结构具有生物形容性与稳定性高、独特的光学性质等优点，使得其在生物成像与活细胞内传感分析等方面非常重要。本项目拟采用仿生识别分子与神经递质共存的纳米探针，通过仿生识别，构筑具有特定构型的等离子纳米超结构，开展结构与拉曼散射信号的性能研究，构建数理模型；通过对等离子纳米超结构的酶稳定性及细胞毒性分析，筛选生物友好型的等离子纳米超结构，在此基础上，重点研究活的神经母细胞瘤分化及活细胞内神经递质与受体互作的表面增强拉曼散射等光谱学研究，实现神经母细胞瘤分化及神经递质与受体互作等的原位实时动态分析，绘制神经递质与受体互作网络，将为神经细胞内电子传递及阿尔茨海默病的早期防治和发病机制等研究提供多角度、系统性的指导。

中文关键词： 等离子纳米超结构；神经母细胞瘤分化；纳米粒子；表面增强拉曼散射；实时分析

英文摘要： Owning to the excellent biocompatibility and stability and unique collective optical properties, plasmonic nanosuperstrucutres could play a key role in the bioimaging and sensing in live cells. This project will construct plasmonic nanosuperstructures with specific configuration via molecular recognition assembled by individual nanoparticles co-modified with DNA and neurotransmitter. And, the project will develop the relationship between the nanoparticle superstructures and the surface Raman scattering signal to build the mathematical model. Furthermore, the project will select plasmonic superstructures with the super biocompatibility through doing the enzyme-tolerance and cytotoxicity experiments. And then, the project will focus on the differentiation of live neuroblastoma and the interaction between the neurotransmitter and receptors in the live cells. Soon afterwards, we will develop a real-time, dynamic and in situ method to analyze the differentiation of live neuroblastoma and the interaction between the neurotransmitter and receptors and draw the interacted profile between the interacted neurotransmitter and receptors. This project will provide a multi-angled and systematic directions for the electron transfer in the neuron cells and the early prevention and pathogenesis for Alzheimer's disease.

英文关键词： Plasmonic nanostructures；Neuroblastoma differentiation；Nanoparticles；Surface enhanced Raman scattering；Real-time analysis