大蒜化感物质消减番茄连作障碍的生理和分子机制

项目名称： 大蒜化感物质消减番茄连作障碍的生理和分子机制

项目编号： No.31471865

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 程智慧

作者单位： 西北农林科技大学

项目金额： 85万元

中文摘要： 连作障碍是设施果菜生产面临的突出问题和制约因素。我们前期研究发现，套作大蒜可以消减番茄连作障碍，并已从大蒜根系分泌物和秸秆中鉴定出主要化感物质二烯丙基二硫（DADS），但其作用机理尚不清楚。本项目用DADS处理番茄，分析其生理响应；通过RNA-seq测定番茄根系基因表达谱，分析番茄响应大蒜化感物质的敏感代谢途径和主效基因及其功能，通过qRT-PCR分析主要相关基因的时空表达特性；利用RNAi和超表达初步分析主效基因（TAO2）的功能及其与大蒜化感物质响应的关系；通过GC-MS鉴定DADS对番茄根系分泌物成分尤其是自毒物质的影响；比较连作土壤条件下DADS对转TAO2基因和非转基因番茄的生长效应，阐明大蒜化感物质消减番茄连作障碍的生理和分子机制。研究结果将为开发大蒜化感物质及应用生理和分子调控技术消减果菜连作障碍奠定理论依据和技术基础，对设施果菜安全可持生产具有重要意义。

中文关键词： 果菜连作障碍；大蒜化感物质；番茄转录组；二烯丙基二硫；基因功能

英文摘要： Continuous cropping obstacles are the obvious problems and constraints faced by fruit vegetables production in greenhouse. We have found that the continuous cropping obstacles of tomato can be alleviated by intercropping with garlic, and diallyl disulfide (DADS) identified is the major allelochemical in garlic root exudates and garlic straws, but its physiological and molecular mechanisms are still unclear. In this project we will study the physiological effect of diallyl disulfide on tomato, investigate the allelopathy of diallyl disulfide on tomato root genes expression profiles by RNA-seq, analyse the sensitive metabolic pathways and functions of genes that response to garlic allelochemical and confirm the temporal and spatial expression characteristics and transcription level of major genes by qRT-PCR. We also want to analysze the corelation between the major gene (TAO2) and diallyl disulfide by RNA interference and overexpression technology. The impact of diallyl disulfide on tomato root exudates and especially the autotoxins changes will be studied by GC-MS. Then the growth effect of diallyl disulfide on TAO2 transgenic and non-transgenic tomato plants in the continuous cropping soil will be analysed. These researches will clarify the physiological and molecular mechanism of garlic allelochemical diallyl disulfide in alleviating tomato continuous cropping obstacles, in order to lay the theoretical basis and technological foundation for the development of garlic allelochemicals and the application of physiological and molecular regulation in alleviating fruit vegetable continuous cropping obstacle.?It is of great significance for the safe and sustainable development of fruit vegetables production in greenhouse.

英文关键词： continuous cropping obstacles of fruit vegetables;garlic allelochemical;tomato RNA-seq;diallyl disulfide;gene function