膳食、血浆及红细胞脂肪酸与中老年人动脉粥样硬化关系的6年前瞻性研究

项目名称： 膳食、血浆及红细胞脂肪酸与中老年人动脉粥样硬化关系的6年前瞻性研究

项目编号： No.81472965

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 陈裕明

作者单位： 中山大学

项目金额： 60万元

中文摘要： 脂肪摄入总量及脂肪酸（FA）类型是影响动脉粥样硬化（AS）性心脑血管病（CVD）的重要因素。国外对传统的饱和、单不饱和及多不饱和FA(尤其是n3系列）及其比例对AS或CVD的影响研究较多，但对FA适宜比例或模式及内生FA的研究较少。迄今，修订我国居民脂肪DRI主要依据国外资料，国内尚无大型前瞻性研究来验证不同FA对AS的作用，也未能确立不同FA的适宜比或总体模式。为此，本研究拟利用2008-10年建立的3千余名40-75岁广州社区居民队列，于第0、3和6年三次调查，通过6年的前瞻性研究，验证及比较膳食、血浆和红细胞膜不同种类、比例及模式的FA对颈动脉内中膜厚度6年变化及AS发生风险的作用，确立该人群FA各大类及亚型的适宜摄入量和适宜比例及总体模式，为修订我国居民DRIs提供科学依据；通过路径分析，检验血管内皮功能、炎性反应、氧化应激、血脂和体脂在FA影响AS途径中的作用以阐明其作用机制。

中文关键词： 脂肪酸；动脉粥样硬化；中老年人；前瞻性研究

英文摘要： Total fat intake and different types of fatty acids (FAs) have been proposed to play an important role in the development and progress of atherosclerosis (AS). Many large-scale studies have evaluated the effects of traditional saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), n-3 or n-6 polyunsaturated fatty acid (PUFA), and their ratios (i.e., SFA:MUFA:PUFA) on progression of AS in European countries, however, scare studies were conducted in Chinese. In addition, few studies have focused on FA patterns and FAs in de novo lipogenesis pathway. Up to date, the dietary recommandance intakes (DRIs) of fat and fatty acids for Chinese residents were made mainly based on data from foreign countries which have different diet habits, and no large prospective studies have assessed the effects of different (or types or subtypes of) FAs, or FA patterns on the risk of AS in Chinese. To address these issues, we proposed this study based on a community-based cohort of 3216 Guangzhou residents (40-75y) established during 2008-2010, subjects completed baseline survey and first follow-up during 2011-2013 (2564 subjects) and will complete the second follow-up during 2014-2016 (~2100 subjects). Nutritional and dietary factors would and will be collected using food frequency questionnaire and blood or 24-h urinary analyses. Dietary consumption and blood (plasma and erythrocytes) composition of 27 types of FAs were/will be assessed at baseline and the followups. Inflammatory cytokines, oxidative/anti-oxidative factors, blood lipids and body fat composition at each visit will be determined. The intima-media thicknesses (IMTs) of common carotid artery (CCA) and bifurcation (BIF) and endothelial function (FMD) were determined via B-mode ultrasound with a high-resolution, 7.0-12.0 MHz linear-array transducer system (Aplio TOSHIBA, Japan). Univariate and multivariate analyses were used to determine the associations of various FAs, FA patterns, blood inflammatory cytokines and oxidative/anti-oxidative factors with the IMT changes in 6-y and risk of AS , and these results will provide the scientific evidence for making the reasonable DRIs of different types of FAs for the AS prevention in Chinese. To address the potential mechanism of AS, path analysis will be used to determine the role of endothelial function, inflammatory cytokines, oxidative stress, blood lipids and body fat composition in the causal chain between FAs and IMT changes or risk of AS.

英文关键词： fatty acids;atherosclerosis;middle-aged and elderly;prospective study