石墨烯-有机共轭分子纳米墙与纳米线电极直接、自由转换检测不同靶基因的比较研究

项目名称： 石墨烯-有机共轭分子纳米墙与纳米线电极直接、自由转换检测不同靶基因的比较研究

项目编号： No.21275084

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 杨涛

作者单位： 青岛科技大学

项目金额： 80万元

中文摘要： 本项目拟构建新颖的石墨烯-有机共轭分子纳米墙与纳米线，其具有丰富的共轭结构、大量电活性位点与优异导电性能可实现直接、高灵敏、自由转换检测转基因植物产品不同靶基因。利用石墨烯特性（如二维共轭单原子层）和有机共轭分子特性（丰富的共轭结构、各种特色功能团、优异电化学活性），采用化学法和电化学法设计一系列复合纳米墙、线界面，对比各种制备方案、实验参数对界面形貌、尺寸、电化学信号等的影响。借助共轭界面与DNA碱基相互作用，实现DNA在电极表面有效非共价固定（后续自由转换检测的基础）；探讨纳米界面自身电化学性质受DNA固定与杂交的影响（"信号开、关"）；对比研究不同形貌、间隙、尺度纳米墙与纳米线电极在DNA固定、杂交效率、灵敏度以及再生性能的差异，研究石墨烯-有机共轭分子纳米结构电极协同改善传感器分析性能的机理及规律。将制备的电化学传感器直接、高灵敏、自由转换检测转基因植物产品的不同靶基因片断。

中文关键词： 石墨烯；有机共轭分子；纳米墙；直接检测；自由转换检测

英文摘要： In this project, novel graphene-organic conjugated molecule nanowall and nanowire electrodes, which possess rich-conjugated structures, plenty of electrochemical sites, and super conductivity, will be carried out for direct, highly sensitive, and freely switchable detection of different target genes originated from real sample of the transgenic plants. Chemical and electrochemical routes will be adopted to prepare a series of nanowall and nanowire nanocomposites, which integrate graphene (a single-atom thick, two-dimensional sheet of sp2 bonded conjugated carbon) with organic conjugated molecule (owning rich-conjugated structures, functional groups, and excellent electrochemical activity). We will study the effect of different preparation schemes and experimental parameters on the morphology, size, and electrochemical signals of the nanocomposites. The immobilization of the probe DNA will be mainly conducted via noncovalent methods (for freely switchable detection) due to π-π interaction between conjugated nanocomposites and DNA bases. The hybridization between the probe DNA and target DNA induces the formation of dsDNA. The resulted dsDNA will release from conjugated nanocomposites, accompanied with the self-signals changes of nanocomposites (signal-on and signal-off). The self-signals changes will serve as a p

英文关键词： graphene；organic conjugated molecule；nanowall；direct detection；freely changeable detection