基于钌-稀土异多核配合物的近红外荧光探针构建及生物成像中应用研究

项目名称： 基于钌-稀土异多核配合物的近红外荧光探针构建及生物成像中应用研究

项目编号： No.21301034

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 徐丽

作者单位： 广东药学院

项目金额： 24万元

中文摘要： 稀土近红外发光探针因具有特殊的荧光性质在生物荧光成像领域具有重要的应用前景。因有机配体敏化稀土离子近红外发光的激发波长主要位于近紫外区而限制其在生物医学领域的应用。因此设计合成采用可见光激发过渡金属发色团来敏化稀土近红外发光且适用于生物荧光成像的新型近红外荧光探针分子将成为这一领域的研究热点。本项目拟以多吡啶配体为桥联配体，在辅助配体上引入靶向基团，构筑一系列新型钌-稀土异多核金属配合物。评价配合物在不同条件下的近红外发光性能，从中筛选出发光性能优异的配合物将其应用于细胞和动物荧光成像研究，阐明其结构与性质的关系，并深入探讨作用机理。本项目的实施将为稀土近红外发光探针在生物成像领域的研究和应用提供新的设计思路和理论依据。

中文关键词： 金属配合物；近红外；荧光探针；生物成像；抗肿瘤

英文摘要： Lanthanide near-infrared luminescent probes have important application prospects due to their unique luminescence properties in the field of biological fluorescence imaging. The excitation wavelength of near-infrared emitting from lanthanide ions sensitized by organic ligand locates mainly in the near ultraviolet region which limits its application in biomedical field. Therefore the design and synthesis of a series of novel near-infrared luminescent probes using visible light to excite transition metal chromophores to generate sensitized luminescence from near-infrared emitting lanthanide(III) centres and being suited to use in the field of biological fluorescence imaging will become the focus of research in this field. In this project, we will construct a series of new ruthenium-lanthanide heteropolynuclear complexes using the pyridine ligands as the bridging ligands and modifying the auxiliary ligands with the targeting group. Near-infrared luminescence properties of these complexes will be studied under different conditions, to screen out some complexes with preferable optical property for cell and animal imaging studies. The relationship between the structure and properties will be clarified, and the interaction mechanism will be further discussed. The implementation of the project will provide a new strateg

英文关键词： metal complexes；near-infrared；luminescent probes；bioimaging；anticancer