基于荧光分子释放的DNA水凝胶的真菌毒素适配体超灵敏传感器的研究

项目名称： 基于荧光分子释放的DNA水凝胶的真菌毒素适配体超灵敏传感器的研究

项目编号： No.31301495

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 周璐

作者单位： 中国科学院苏州纳米技术与纳米仿生研究所

项目金额： 21万元

中文摘要： 近年来世界范围内屡屡发生的食品安全事件，使食品的安全检测倍受关注，发展快速超灵敏的检测方法成为亟待解决的问题。本项目设计一种智能响应的全DNA水凝胶释放荧光信号分子的适配体传感器，实现检测信号的放大，用于食品中的赭菌霉素OTA和黄曲霉素AFT的超灵敏检测。将靶分子适配体序列与骨架DNA序列组装成全DNA水凝胶，同时荧光信号分子封装在其中，再由靶分子识别来调控释放封装的大量荧光信号分子，达到少量靶分子释放大量信号分子的放大过程，从而实现对靶分子的超灵敏检测。重点研究全DNA水凝胶的设计和组装，考察其包埋荧光信号分子的能力以及对靶分子的响应敏感度，以及荧光信号分子的尺寸对检测背景及其释放过程的影响，以揭示由全DNA水凝胶中释放荧光信号分子的信号放大方法的构建规律和内在机理。本项目发展的超灵敏、简单、快速的信号放大方法具有非常重要的科学意义和应用价值，可成为食品安全检测中超灵敏检测的普适方法。

中文关键词： DNA水凝胶；适配体；赭曲霉素；T4 多聚核苷酸激酶；生物传感器

英文摘要： Since food safety problems attract more and more attention, it is highly desired to develop more sensitive,selective，rapid and convenient detection methods in food safety field.Ochratoxin A and aflatoxin are mycotoxins and naturally occurring toxins produced by certain fungi that can grow on foods such as cereals, nuts, dried fruits, spices, legumes and others under certain environmental conditions.In order to develop an ultrasensitive aptasensor for the mycotoxins,this project is to design the fluorescent signal molecule carrier by pure DNA hydrogel responsing to target toxins.Unlike the conventional hydrogels, the pure DNA hydrogel including the aptamer sequences for target toxins is an ideal carrier for the design of an "on-command" delivery system because of its distinctive characteristics, such as large load capacity, biocompatibility and controllable conformation. In addition ,the DNA hydrogel is totally assembled from oligonucleotide domains with controllable conformation and size.To achieve the signal amplification,the fluorescent molecules are encapsulated by the pure DNA hydrogel and can be released by the stimulus of the target toxins. This stimulated-release process makes it possible for the detection of very low concentration of target by transfering to the aggregated signal by a large number of fl

英文关键词： DNA hydrogel；aptamer；Ochratoxin A；T4 Polynucleotide Kinase；Biosnesor