EGB抗氧化处理促进BMSCs归巢与定向分化胰岛样细胞治疗糖尿病的机制研究

项目名称： EGB抗氧化处理促进BMSCs归巢与定向分化胰岛样细胞治疗糖尿病的机制研究

项目编号： No.81503531

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 任明

作者单位： 吉林大学

项目金额： 18万元

中文摘要： 糖尿病是严重危害人体健康的疾病，因胰岛素分泌不足或利用受阻引起血糖升高,慢性高血糖产生葡萄糖毒性作用,与糖尿病及其并发症的发生发展密切相关。课题组前期研究发现，BMSC在体外经过适当的诱导能够分化为胰岛样细胞并具有分泌胰岛素的功能，但BMSC移植后的低存活率影响其对糖尿病的治疗效果，而经银杏叶提取物(EGB)预先给予治疗后可提高BMSC移植的存活率，降低糖尿病大鼠血糖，其机制尚不清楚。本研究通过构建氧化应激和糖尿病大鼠模型，深入研究EGB、EGB含药血清和氧化应激对BMSC增殖分化为胰岛样细胞的影响；采用血清药理学方法，分析各时间点获得的EGB含药血清有效成分与BMSC增殖分化的关系；重点从蛋白质表达水平上探讨EGB抗氧化治疗增强BMSC治疗糖尿病的机制及与MAPKs信号转导通路的可能联系，为提高BMSC移植治疗效果寻求治疗靶点并为抗氧化在BMSC移植中的应用提供实验学依据。

中文关键词： 银杏叶提取物；骨髓间充质干细胞；糖尿病；胰岛样细胞；氧化应激

英文摘要： Diabetes is a serious harm to human health disease, due to lack of insulin secretion or disruption caused by the use of elevated blood sugar, high blood sugar produces chronic toxic effects of glucose, is closely related to the development of diabetes and its complications. Our previous studies have found that bone marrow mesenchymal stem cells (BMSC) in vitro after appropriate induction and can differentiate into islet-like cells secrete insulin function, but the low survival rate after transplantation BMSC its impact on diabetes therapeutic effect, but after the Ginkgo biloba extract (EGB) pretreated BMSC transplant treatment can improve the success rate, lower blood glucose levels and reverse oxidative stress in diabetic rats, the mechanism is unclear. In this study, we research the affect of EGB, EGB containing serum and oxidative stress on the proliferation and differentiation of BMSC by building oxidative stress and diabetic rat model. Using serum pharmacology methods to analyze the relationship of the active ingredient obtained at each time point of EGB containing serum and BMSC proliferation and differentiation. Focusing on the protein expression levels to explore the mechanisms EGB enhanced BMSC treatment of diabetes and possible communication with MAPKs signal transduction pathways, to improve the therapeutic effect of BMSC transplantation and seek therapeutic target for anti-oxidation in BMSC transplantation application provides a scientific basis.

英文关键词： Ginkgo biloba L extract ;Bone marrow mesenchymal stem cells;Diabetes mellitus;Islet-like cells ;Oxidative stress