海洋新菌株ZC1的琼胶降解酶系统研究

项目名称： 海洋新菌株ZC1的琼胶降解酶系统研究

项目编号： No.41476150

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 海洋科学

项目作者： 胡忠

作者单位： 汕头大学

项目金额： 20万元

中文摘要： 通过主持完成多项国家自然科学基金等项目，申请者课题组开展了琼胶酶的性质、作用机制研究；并发现海洋新细菌ZC1不同于已报道的琼胶降解菌株：1）无琼胶诱导下产生高活性琼胶酶，含有组成型琼胶酶；2）其基因组中存在40个推测琼胶酶编码基因，为目前报道具有推测琼胶酶基因数量最多的菌株；3）对所有推测琼胶酶基因进行功能验证，7个推测琼胶酶基因具有琼胶酶解功能，是已报道含有琼胶酶数量最多的菌株。本项目拟进一步通过基因重组表达和免疫检测等技术，研究菌株ZC1中主要琼胶酶的酶学性质、酶解产物和酶反应动力学等，分析主要琼胶酶在琼胶降解中的作用，初步阐释菌株ZC1的琼胶降解酶系统；预期成果将促进对微生物利用海藻多糖机制的认识，促进海藻多糖的开发利用。

中文关键词： 海洋微生物；琼胶；琼胶酶；酶解系统；

英文摘要： Through accomplishing several research projects about agarase including the National Natural Science Fundation projects, the applicants have much experience of research on agarase properties and their enzymatic mechanisms. Among all the findings, the marine novel strain ZC1 owns the following special characteristics compared to other reported agar-degrading strains: 1) The production of agarases without the induction of agar, indicating the presence of constitutive agarases; 2) the most putative agarase genes in the reported genomes, with the amount of 40; 3) senven agarases belonged to different glucoside hydrolase families were confirmed after all the 40 putative agarase genes were expressed in E.coli, so strain ZC1 owns the most confirmed agarases agarases of strain ZC1 and study their cellular location and time course of expression using RT-PCR and western bloting, and finally illuminate the agarases' roles and the enzyme system in the agar degradation by strain ZC1 combined with the enzymatic properties of those confirmed agarases. At the mean time, the research on the agarase system will also provide references for the prediction of the agarase systems of other agar-degrading strains based on the comparisons of functional domians composition and regulation sequences of agarase genes. This project will prom

英文关键词： Marine microbe；agar；agarase；enzymatic system；