基于微流控平台的载药纳米颗粒联合间充质干细胞双载体对脑胶质瘤靶向效应研究

项目名称： 基于微流控平台的载药纳米颗粒联合间充质干细胞双载体对脑胶质瘤靶向效应研究

项目编号： No.31500793

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 马静云

作者单位： 大连医科大学

项目金额： 20万元

中文摘要： 脑胶质瘤具有弥散性生长及放化疗高度抗性，运用目前手术切除-放化疗联合疗法难以对其根治，亟需发展新型高效治疗体系；基于间充质干细胞（MSCs）的肿瘤趋化特性，以MSCs为载体的靶向胶质瘤治疗显示了独具优势的前景。目前相关研究尚无明确结论，存在模型体系与体内真实微环境偏差大、终点分析难以实时动态监测细胞行为的重要缺陷。本项目拟以极具潜力的微流控芯片为核心技术，充分利用该技术细胞可控三维培养、流体精确操控等优势，在前期功能化芯片制备、胶质瘤三维微环境构建及药物载体材料合成的工作基础上，以胶质瘤细胞和MSCs为研究对象，兼顾细胞-细胞、细胞-细胞外基质等三维微环境作用机制，创新性地建立和发展基于微流控技术的MSCs胶质瘤靶向作用整合模型新体系，并探索载药纳米颗粒联合MSCs双载体对胶质瘤靶向效应芯片应用研究，从而建立一种体外胶质瘤治疗评价新技术、新平台，解决现阶段胶质瘤治疗面临的瓶颈问题。

中文关键词： 微流控芯片；脑胶质瘤；间充质干细胞；载药纳米颗粒；靶向效应

英文摘要： Gliomas possess properties of diffuse growth and high resistant to chemoradiotherapy. Currently, it is still difficult to cure gliomas by using combination of surgery and chemoradiotherapy. New and efficient treatment systems are urgent to be developed for glioma therapy. Based on the tumor tropism in mesenchymal stem cells (MSCs), targeted glioma therapy using MSCs carrier shows unique advantages and prospects. However, there is no clear conclusion from related research so far, and the disadvantages of the existing models include deviation between the model system and the in vivo microenvironment, difficulties in dynamic detection of the cell behaviors in endpoint analysis. This project plans to utilize microfluidic chip as the core technology, taking full advantage of microfluidic technology in controllable 3D cell culture and precise fluid control, based on previous research work on functional chip preparation, 3D glioma microenvironment construction and drug carrier material synthesis, using the glioma cells and MSCs as objects of study, combining intensely investigating the cell-cell, cell-matrix interaction in the microenvironment, to establish and develop innovative integrated model system for stem cell based glioma targeting and explore the application of targeting effect of drug loaded nanoparticle-mesenchymal stem cell double carriers to glioma on the microfluidic platform. The project aims to establish a novel and effective technology and platform for glioma treatment and evaluation in vitro, to solve the bottleneck problems in current glioma therapy.

英文关键词： Microfluidics;Glioma;Mesenchymal stem cells (MSCs);Drug-loaded nanoparticles;Targeting effects