PKC/MAPKs信号途径在BDE-47致菲律宾蛤仔血细胞损伤中介导机制的研究

项目名称： PKC/MAPKs信号途径在BDE-47致菲律宾蛤仔血细胞损伤中介导机制的研究

项目编号： No.31302166

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 刘静

作者单位： 温州医科大学

项目金额： 20万元

中文摘要： PBDEs是一类新型持久性有机污染物，其污染已扩展到海洋生物中。近年来研究表明污染物能导致贝类免疫力下降，增加对病原的易感性，但缺乏污染物对贝类免疫毒性机制的深入研究。故本项目针对这一薄弱环节展开研究，以BDE-47为污染物，首先观察BDE-47对菲律宾蛤仔血细胞吞噬率、抗菌活力、活性氧、血细胞损伤（肌动蛋白、溶酶体膜稳定性、脂质过氧化、DNA损伤）以及PKC/MAPKs激酶表达的影响，确定BDE-47对血细胞免疫毒性的时间剂量效应及PKC/MAPKs信号通路的时空表达；其次，分别利用PKC、ERK、JNK、p38与ROS抑制剂分别阻断其表达，然后观察PKC/MAPKs通路分别被抑制后血细胞免疫力与血细胞损伤的变化，以确定PKC/MAPKs通路在免疫损伤调节过程中各自的作用靶点，同时查明PKC、MAPKs与ROS之间的对话关系，从而为揭示BDE-47对贝类的免疫毒性机制提供科学依据。

中文关键词： 菲律宾蛤仔；血细胞；免疫损伤；凋亡；信号通路

英文摘要： PBDEs is a new type persistent organic pollutant, now the pollution scope has expanded to halobios. In recent years, researches have shown that POPs could cause the decrease of immune ability and increased susceptibility to disease microbes, however it is still lack of the immutoxicology mechanism. Therefore in this program we study the immunotoxicity mechanism of BDE-47 on the haemocyte of clam （Ruditapes philippinarum). First, we study the effects of BDE-47 on the phagocytic activity，bacteriolytic activity，ROS，haemocyte damge (actin filament disruption, lysosomal membrane stability, lipid peroxidation and DNA damage) as well as PKC/MAPKs kinase expression in order to make sure the dose- and time-response of haemocyte damage exposed to the BDE-47, also do the PKC/MAPKs signaling pathways. Next we use the inhibitors of PKC、ERK、JNK、p38 and ROS to block the signaling pathways of PKC/MAPKs respectively, then observe the changes of the immune defence parameters and the haemocyte damage, so we can determine the acting targets of the PKC/MAPKs. Meanwhile we also can find out the "cross-talk "of the PKC, MAPKs and ROS. The results will provide more scientific basis about the immunetoxicity mechanism of BDE-47.

英文关键词： Ruditapes philippinarum；haemocyte；immune damage；apoptosis；cell signaling