电色谱微流控芯片关键技术研究

项目名称： 电色谱微流控芯片关键技术研究

项目编号： No.20805007

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 王伟

作者单位： 福州大学

项目金额： 20万元

中文摘要： 本项目将新兴的电色谱分离技术和微流控芯片结合起来，研究制备有机整体柱和无机硅胶整体柱电色谱微流控芯片，对制得的整体柱芯片进行性能表征，发现芯片具有良好分离性能和稳定性。完成了电色谱芯片关键技术中，泵压、高电压引入接口的研制，完成了电色谱芯片中进样和分离流路的设计，针对不同的检测方式，设计了电色谱芯片安培检测接口和电色谱芯片激光诱导荧光检测接口。应用前面工作中完成的安培检测接口和激光诱导荧光检测接口，在电色谱芯片上进行了氨基酸、多肽等生化样品的分离检测，结果表明，接口具有良好的稳定性和应用性。此外，针对传统芯片通道较细，负载小的特点，对宽而浅的薄膜状的芯片通道也进行了研究，取得了满意的效果，并正将其应用于电色谱通道的制备。项目研究解决了电色谱芯片研究技术中的关键性问题。

中文关键词： 微流控芯片;电色谱;电色谱芯片;整体柱

英文摘要： This project combined technologies of newly arised electrochromatographic separation and microfluidic chip. The conditions to fabricate polymer monolithic column and inorganic silica monolithic column microfluidic chips were studied in detail. The fabricated microchips were found to be stable and had high ability for separation. The interfaces to introduce pump pressure and high voltage into microchip were designed. Furthermore the injection and separation channel patterns were optimized to improve the function of microchip. Biochemical sample, such as amino acids and peptides were separated and determined on electrochromatographic microfluidic chip with newly designed interfaces of amperometric detection and laser induced fluorescence detection. The result showed that the interfaces were stable and applicable. Filmy channel was also studied to solve the problem of low sample load caused by narrow channel in commercial microchip and it is applied to electrochromatographic channel now with satisfactory results. The study of project solved the key technologies of electrochromatography microchip research.

英文关键词： Microfluidic chip; Electrochromatography; Eelectrochromatography microchip; Monolithic column