南海岛屿典型植物内生真菌代谢组差异扫描及新活性物质发现

项目名称： 南海岛屿典型植物内生真菌代谢组差异扫描及新活性物质发现

项目编号： No.21502197

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 环境科学、安全科学

项目作者： 李翰祥

作者单位： 中国科学院华南植物园

项目金额： 21万元

中文摘要： 特殊生境真菌的新活性物质发现几率往往较高，其中，海洋岛屿生态系统因海域隔离，显著有别于其他生态系统。然而，目前海岛植物内生真菌次生代谢物的研究却很少有报道。申请者等的前期工作对南海三种典型海岛植物调查，从中分离得到127株真菌，本项目着力于如何快速从这些真菌中发现新结构活性物质，通过基于代谢组差异扫描设计思路，寻求新天然有机小分子发现方法上的突破。拟对获得菌株不同培养条件少量发酵，运用HPLC-DAD/MS和2D NMR等技术进行代谢组差异分析；结合天然产物数据库排重结果，对培养条件敏感、指纹图谱特殊的菌株放大发酵，跟踪分离鉴定这些差异表达的化合物及其类似物；进行抗炎、抗肿瘤和抑菌等活性测试，获得新活性化合物并探讨构效关系。本项目的完成将为新活性先导化合物的发现提供方法借鉴，同时也为海洋岛屿真菌资源进一步系统性研究奠定基础。

中文关键词： 内生真菌；次生代谢物；代谢组；活性物质；海洋岛屿

英文摘要： Fungi derived from special habitats have been shown in recent years to produce a plethora of new bioactive secondary metabolites. Due to geographical isolation, oceanic island ecosystems are vastly different from other ecosystems. However, endophytic fungi living the special niche, especially their secondary metabolites, have rarely been investigated. In a previous study, 127 endophytic strains have been isolated from three representative plants in South China Sea Islands, indicating that islands plants are a rich source of endophytes. Thus, exploring and mining the fungal potential metabolic capabilities, providing useful bioactive compounds, would be highly desirable. The main purpose of this project is to isolate new bioactive compounds and analogues from the endophytic fungi through metabolome differential scanning. Firstly, the endophtic fungal metabolome will be explored using a small-scale fermentation procedure with different kinds of media combined with HPLC-DAD/MS, and 2D NMR. Secondly, based on the results of dereplication, the endophytes sensitive to culture medium with interesting metabolic fingerprinting will be large-scale cultured; the differentially expressed compounds and the analogues will be isolated and identified. Finally, the bioactivities of isolated compounds such as anti-inflammatory, antitumor or antibacterial property, together with structure-activity relationships will be studied. The results of the project will provide scientific information for the discovery of new lead compounds. In addition, it will establish foundation for the further systematic research on oceanic islands fungi.

英文关键词： Endophytic Fungi;Secondary Metabolites;Metabolome;Bioactive Compounds;Oceanic Islands