自由基诱导DNA损伤的石墨烯纳米电化学分析

项目名称： 自由基诱导DNA损伤的石墨烯纳米电化学分析

项目编号： No.21305046

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 王颖

作者单位： 华东理工大学

项目金额： 25万元

中文摘要： 自由基广泛存在于生物体内，是生命体多种反应的重要参与物质。近年来的研究表明自由基与生命和疾病具有非常紧密的关系，是DNA损伤的致病因素之一。随着人类社会的发展，越来越多的污染源成为自由基损伤DNA的诱发因子，引发严重的疾病和遗传特征的改变。因此，有关自由基诱导DNA损伤的检测和分析研究在疾病诊断、药物开发以及化学试剂的环境评价方面具有重要的意义和研究价值。本项目采用电化学分析技术建立高灵敏的自由基诱导DNA损伤纳米分析体系，借助新型二维纳米材料石墨烯优良的电化学性能和独特的DNA组装效应构建纳米电化学分析界面，通过Feton反应模拟羟基自由基对目标DNA的损伤，建立高灵敏、高准确率的自由基诱导DNA损伤纳米电化学检测平台。本项目将建立基于石墨烯纳米电化学DNA损伤检测的新方法，有望深入探索自由基对DNA损伤的机制原理，为DNA损伤研究注入新的血液，为有关生命安全和人类健康的研究贡献力量。

中文关键词： DNA损伤；电化学；自由基；石墨烯；信号放大

英文摘要： The biological function of free radicals in the organism so far is ambiguous. On the one hand, free radicals can assist the immune system, mediate cell signaling, and be essential in apoptosis. On the other hand, excess free radicals are responsible for causing DNA oxidative modifications and DNA damage, which can initiate carcinogenesis and may play a role in the development of several age-correlated degenerative diseases. Consequently, measurements and clarification of free radical induced DNA damage is highly required for disease diagnostics, medicinal development, as well as environmental assessment. We will carry out the project by preparation of functional graphene nanomaterials with remarkable-performances to build up highly active and efficient biorecognition electrode interface. Based on the unique self-assembly effect of single strand DNA on graphene surface, we could construct the nano-electrochemical analysis platform with high sensitivity and selectivity. DNA damage induced by radicals will be realized through Feton reaction, and electrochemical detection will be accomplished on the constructed DNA-graphene biosensing scaffold. The current project will propose a fresh strategy for free radical induced DNA damage detection and determination, will explore the interaction between free radicals and geno

英文关键词： DNA damage；electrochemistry；radicals；graphene；amplification