微流控芯片—毛细管电泳—微液滴喷射雾化器等离子体质谱联机进行单细胞内金属形态分析的研究

项目名称： 微流控芯片—毛细管电泳—微液滴喷射雾化器等离子体质谱联机进行单细胞内金属形态分析的研究

项目编号： No.21275038

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 刘金华

作者单位： 杭州师范大学

项目金额： 80万元

中文摘要： 单细胞中金属元素的形态分析对提高从正常细胞中辨别不正常细胞的能力，研究金属在细胞中代谢和迁移转化规律等有重要意义。目前尚稀见单细胞中金属元素形态分析的报道。本申请首次提出用喷墨打印头研制微液滴喷射雾化器，以及进一步研制以微液滴喷射雾化器作为接口的CE-ICP-MS联机装置。使ICP-MS雾化器的液流速度与CE电渗流的流速相匹配，消除传统联机系统中补充液对样品的稀释作用和电泳谱带增宽，消除ICP-MS气动雾化器对电泳分离的自吸作用，显著降低CE-ICP-MS的绝对检测下限，提高CE-ICP-MS联机系统进行单细胞中金属形态分析的灵敏度和分辨率等分析性能。在此基础上，进一步通过用微流控芯片操纵单细胞，研制微流控芯片-CE-ICP-MS联机装置，建立快速进行单细胞内金属元素形态分析的方法，研究金属元素在细胞中的代谢规律和细胞个体中金属形态的差异。在金属组学和代谢组学的研究中有广泛的应用前景。

中文关键词： 等离子质谱；电泳；液相色谱；生物样品；元素形态分析

英文摘要： Metal speciation in single cells has great significance for increasing the ability of discriminating abnormal cells from the healthy ones and studying metal transformation and metabolites in cells. However, up to now, reports on metal speciation in single cell are scarce. The aim of the research proposal is achieving rapid speciation analysis of metal in single cells by developing an inkjet-based micro droplet aerosol generator interfacing capillary electrophoresis (CE)- inductively coupled plasma mass spectrometry (ICP-MS) with microfluidic chip single cell injection. The inkjet-based micro droplrt aerosol generator can be operated with high nebulizing efficiency at the low sample uptake rate as the electroosmotic velocity in CE without the use of nebulizing gas. Consequently,the make-up solution used in conventional CE-ICP-MS and suction effect from the conventional pneumatic nebulizer were eliminated in the proposed CE-ICP-MS system. For this reason, the absolute detection limit of the proposed CE-ICP-MS system was significantly reduced with better sensitivity and resolution. On this basis, a hyphenated system, which consists of microfluidic chip single cell manipulation, capillary electrophoretic separation and inductively coupled plasma mass spectrometry detection, will be developed for rapid single-cell m

英文关键词： inductively coupled plasma mass spectrometry；electrophoresis；liquid chromatography；biological samples；elemental speciation analysis