多功能聚羧酸-NHS酯改性溶液态胶原及其构效机理

项目名称： 多功能聚羧酸-NHS酯改性溶液态胶原及其构效机理

项目编号： No.21306024

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 化学工业

项目作者： 张敏

作者单位： 福建农林大学

项目金额： 25万元

中文摘要： 交联改性是当今广泛采用的用于改善胶原稳定性的方法。但目前研究基本集中于固态胶原的改性，这是由于液态胶原的交联改性尚存在两个问题：一是胶原需经酰化改性以改善生理条件下水溶性，无剩余氨基供交联反应使用，二是交联改性过程中胶原易凝胶化。本项目从统筹解决的角度出发，拟合成聚羧酸-NHS酯作为新型胶原改性剂，实现胶原稳定性、水溶性和流动性的同步改性调控。通过构建胶原热变性动力学模型，研究改性对稳定性的影响；通过考察胶原在生理条件下的溶解行为，研究改性对水溶性的影响；通过考察胶原的流变性能、构建相关流变学模型，并揭示交联致胶原凝胶化的机理，研究改性对流动性的影响并实现凝胶化行为有效调控。在此基础上，结合改性对胶原分子结构、聚集态结构的影响机制，最终阐明多功能化改性液态胶原的构效机理。本项目力图突破传统改性思路，研发一类多功能胶原改性剂，对于拓宽液态胶原应用范围、推动动物生物质高值利用进程具有重要意义。

中文关键词： 水溶性胶原；γ-聚谷氨酸；热稳定性；交联作用；细胞毒性

英文摘要： Cross-linking modification is a method which is widely used in the improvement of collagen stability. However, most of the relevant studies focus on the modification of collagen which is in solid state, mainly because there are two problems in the cross-linking modification of collagen in liquid state: the first problem is that collagen has been endured by acylated modification in order to obtain a good water-solubility under the physiological conditions, therefore there is no enough amino-group in collagen molecules for cross-linking reaction, since amino-group has been used up by acylated modification. The second problem is that collagen solution is liable to gelation in the process of cross-linking modification. From the point of view of solving these problems in an integrated manner，in the present project we are planning to synthetize polycarboxylic acid-NHS esters as a novel modification agent for collagen in liquid state. The stability, water-solubility and flowability of collagen solutions could be modified and controlled synchronously by the use of this novel modifier. By building the model of denaturation kinetics of collagen in the process of heating, the effect of modification on the stability of collagen will be investigated. By exploring the dissolution behavior under physiological conditions，the ef

英文关键词： Water-soluble collagen；γ-poly(glutamic acid)；Thermal stability；Cross-linking effect；Cytotoxicity