AtCHYR1在ABA依赖的逆境胁迫途径中的功能研究

项目名称： AtCHYR1在ABA依赖的逆境胁迫途径中的功能研究

项目编号： No.31300995

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 张彬

作者单位： 中国科学院植物研究所

项目金额： 22万元

中文摘要： ABA依赖的逆境胁迫响应途径是植物感受和应答非生物胁迫的重要方式。其分子机制的研究已经取得一定进展，但其精细的调控网络尚不完整。我们在拟南芥中发现一个受ABA和干旱诱导的基因，其编码蛋白具有CHY锌指和RING结构域且功能未知，命名为AtCHYR1。序列分析发现其同源基因也存在于水稻和玉米基因组中且功能未知。初步研究表明，AtCHYR1蛋白具有泛素连接酶活性；其突变体在种子萌发阶段对ABA信号不敏感。因此AtCHYR1可能是植物响应逆境胁迫和ABA信号途径中一个新的重要因子。本项目将通过分子、生化、细胞生物学等手段研究AtCHYR1在ABA依赖的逆境胁迫响应中的生物学功能；通过免疫共沉淀、蛋白质组技术鉴定其互作蛋白；通过体外泛素化反应，阐明AtCHYR1与其互作蛋白的关系，最终确定其在植物细胞内发挥功能的分子机制。本研究将揭示植物中CHYR类蛋白在逆境信号传递中的作用，加深对该过程的认识。

中文关键词： 干旱耐受性；气孔运动；泛素化；磷酸化；SnRK2.6

英文摘要： In plants, ABA-dependent stress-responsive pathway plays an important role in sensing and responding to the abiotic stress signal, and its molecular mechanism has been extensively explored. To date, however, the regulatory network is still largely incomplete. Here we found a novel gene up-regulated during ABA and drought treatments, which encodes a protein containing both a functional-unknown domain CHY-zinc finger and a RING finger domain, designated as AtCHYR1 (Arabidopsis thaliana CHY-zinc finger and RING finger containing 1). Sequence analysis showed that several potential AtCHYR1 ortholog genes were found in rice and maize, with yet unknown functions. Preliminary results indicated that AtCHYR1 possessed E3 ubiquitin ligase activity and the AtCHYR1 T-DNA insertion mutants were insensitive to ABA during seed germination. Above all, these results suggest that AtCHYR1 may participate in the ABA and stress-responsive pathway as a new important factor. In this study, we will employ molecular genetics, biochemical and cellular biology manners to characterize the function of AtCHYR1 in ABA-dependent stress-responsive pathway. Besides, we are also interested to find the interaction factor of AtCHYR1 via co-immunoprecipitation and proteomics approaches and clarify their relationship through in vitro ubiquitination as

英文关键词： drought tolerance；stomatal movement；ubiquitination；phosphorylation；SnRK2.6