基于ATP途径的能量生物材料构建及其促进骨愈合的分子机理研究

项目名称： 基于ATP途径的能量生物材料构建及其促进骨愈合的分子机理研究

项目编号： No.81471792

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 张胜民

作者单位： 华中科技大学

项目金额： 80万元

中文摘要： 生物能量分子三磷酸腺苷（ATP）对促进细胞生长增殖、提高成骨活性具有重要影响。然而，由于ATP分子的不稳定性，如何实现含ATP能量生物材料的构建仍面临巨大挑战。目前尚未见该类能量生物材料应用于组织构建和再生医学的报道。本申请基于课题组成员前期在含能生物材料构建和ATP的稳定化固载技术领域的一项新进展，为破解上述难题提供了可能。主要研究内容包括：含能生物材料的分子设计与构建；ATP在多尺度空间支架上的活性化固载及其可控释放研究；新材料的体外评价与体内骨缺损修复再生；含能生物材料促进细胞增殖与提高成骨活性的作用途径及调控规律研究等。通过上述具体和关键科学问题研究，获得对含能生物材料促进骨再生的分子机制及其调控规律的深刻理解和认识，进而为发展应用于骨缺损快速修复的新一代能量生物材料奠定重要理论基础。这将是生物材料与再生医学领域的一个重要创新和进展。

中文关键词： 生物材料；骨修复；成骨分化；能量生物材料；支架材料

英文摘要： Adenosine triphosphate(ATP),a kind of energic biomelecules, has a significant effect on promoting cell growth and proliferation, as well as improving osteogenic activity. However, due to its unstability, fabrication of such ATP-conjugated bioenergic materials remains a huge challenge. So far, there is no paper published on this kind of bioenergic materials applied to tissue construction and regenerative medicine. Recently, a technical breakthrough from our group on fabrication of bioenergic materials and ATP-stabilized conjugation makes it possible to solve the problem above. The research contents mainly include: molecular design and fabrication of bioenergic materials;activited immobilization and controlled release of ATP on muli-scaled 3-D scaffolds; in vitro evaluation, bone defect repairing and regeneration in vivo; studies on pathway, molecular mechnism and regulation rule for bioenergic materials promoting cell proliferation and improving osteogenic activity mediated by extracellular ATP signaling; etc. Through studies on the specific and key scientific issues mentioned above, we expect that the insight understanding and new knowledge of molecular mechanism and regulation rule for bioenergic materials improving bone regeration will be obtained, which will further provide an imprtant theoretical basis for devoloping the next generation bioenergic materials with rapid bone repairing functions. Therefore, the current study will become an important innovation and progress in biomedical materials and regenerative medicine.

英文关键词： Biomaterials;Bone repairing;Osteogenesis differentiation;Bioenergetic materials;Scaffold