南海红树林微生物抗肿瘤活性代谢产物的研究

项目名称： 南海红树林微生物抗肿瘤活性代谢产物的研究

项目编号： No.21272286

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 刘岚

作者单位： 中山大学

项目金额： 80万元

中文摘要： 红树林由于其丰富的生物多样性，存在种类极其丰富的微生物，可产生大量结构多样，具有特殊生理活性的化合物。本课题组已经从一株红树林内生真菌发现了多种种具有抗肿瘤活性的倍半萜类新化合物，并从一株放线菌中发现了一种高抗肿瘤活性低毒性放线菌类新化合物。本课题将继续红树林微生物的研究，一方面继续从南海红树林分离、筛选、鉴定、保藏新的活性微生物菌株，并对高活性菌株进行代谢产物研究，寻找新的活性代谢产物；另一方面根据已有工作基础，重点对这两种具有抗肿瘤活性的微生物进行进一步的研究，通过菌种优化和菌种诱变，一方面提高新化合物产量，供进一步生理活性研究，同时进一步分离分析不同培养条件及诱变条件下的代谢产物，以期从中发现更多新结构化合物,研究其生理活性，寻找新的抗肿瘤药物先导化合物。对已发现新化合物进行结构改造，研究其构效关系，以提高新化合物的抗肿瘤活性。本工作对南海海洋微生物的资源开发利用有重要意义。

中文关键词： 红树林；内生真菌；抗肿瘤；次级代谢产物；

英文摘要： Mangrove can produce large number of microorganisim species because of the biodiversity. The metabolites of the microbes is of great structure diversity and special bioactivity produce with the competition for existence. In previously research,several kinds of new compounds with antitumor acitivity which were found in a mongrove fungus of South China Sea. One new kinds of actinomycin structure with high antitumor activity and low toxicity was found in a actinomycete from mangrove . This project will keep on research on mangrove bioorganism. Firstly, isolate and identificate and preserve the bioactive bioorganism strain from mongrove forest of South China Sea. Study the new bioacitve metabolites of the bioorgnism so as to find new antitumor compounds.Secondly, focus on the two strains with research foundation, to improve the production of bioactive new compound by cultrue optimize and strain mutagism. At the same time ,go on deep researsh on the metabolites under different culture condition and different mutagenesis condition,in order to find new structure and study the bioactive of the compound ,so as to find new leading antitumor drug compounds. Modify the structure of high acitve new compounds, research the structure activity relationship of the compounds，to improve the antitumor activity of the new compoun

英文关键词： Mangrove；Endophytic fungi；Anticaner；Secondary metabolites；