玉蜀黍赤霉有性生殖发育不同阶段特异性转录因子的调控机制研究

项目名称： 玉蜀黍赤霉有性生殖发育不同阶段特异性转录因子的调控机制研究

项目编号： No.31301614

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 林杨

作者单位： 华中农业大学

项目金额： 22万元

中文摘要： 玉蜀黍赤霉（无性态：禾谷镰孢）引起的赤霉病是世界范围内的小麦流行病害。其有性生殖产生的子囊壳是该菌越冬的主要形式。同时有性孢子（子囊孢子）也是该病流行的初侵染源。有性生殖还保持了居群致病力相关基因的遗传多样性和稳定性，从而提高其对宿主植物的适应能力。因此对有性生殖的理解是综合控制赤霉病的先决条件。研究转录因子能将上游信号途径和下游靶基因连接起来。从前期的研究结果中，我们筛选出5个在有性生殖不同阶段（包括调控雌配子育性、子囊壳数量、子囊壳大小、子囊壳成熟、子囊孢子成熟等）特异发挥调控作用的转录因子。本项目拟以这5个转录因子为研究对象，利用染色质免疫沉淀测序技术并结合表达谱测序结果和体外电泳迁移率实验找出它们的候选靶基因。最后对候选靶基因进行功能分析。该研究将首次阐明特异地作用于玉蜀黍赤霉有性生殖不同发育阶段转录因子的基因调控机制，从整体上认知其有性生殖发育表达调控机制，为赤霉病防治提供线索。

中文关键词： 小麦赤霉病；有性态；转录因子；杀菌剂；抗药性

英文摘要： Gibberella zeae (anamorph: Fusarium graminearum) is an important plant pathogen that causes Fusarium head blight (FHB) in wheat throughout the world. Perithecia or associated hyphae are known to be the components of fungi that aid in survival during the winter season. The sexual spores, ascospores, serve as the primary inocula for disease epidemics. Sexual reproduction also helps the G. zeae population maintain genetic diversity and genetic stability. In addition, high genetic diversity of virulence-related genes is thought to provide G. zeae with a great capacity for adaptability toward host plants. Thus, the understanding of sexual development is a prerequisite for integrated control of FHB disease. Studies on transcription factors will enable us to link upstream signaling pathways with downstream target genes. Based on previous studies, we selected five transcription factors that specificlly play regulatory roles in sexual development at different stages (including the regulation of female fertility, the number of perithecia, the size of the perithecia, perithecia mature and ascospores mature). In this project we intend to identify target genes of these transcription factors by performing Chromatin Immunoprecipitation Sequencing（ChIP-Seq）, combining with the results of RNA-sequencing （RNA-Seq）and Electrophore

英文关键词： Fusarium graminearum；Telomorph；Transcription factor；Fungicides；Fungicide resistance