发形霞水母（Cyanea capillata）触手转录组分析及其重要活性因子的克隆表达与功能研究

项目名称： 发形霞水母（Cyanea capillata）触手转录组分析及其重要活性因子的克隆表达与功能研究

项目编号： No.41306136

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 天文学、地球科学

项目作者： 柳国艳

作者单位： 中国人民解放军第二军医大学

项目金额： 27万元

中文摘要： 海洋中数量丰富的水母产生的生物活性物质具有多样性的特点，是水母中毒复杂性的重要基础，也是潜在的海洋药物资源宝库。本课题组前期通过高通量测序，获得了我国东南海域代表性有毒水母--发形霞水母触手转录组并进行了初步分析，发现其中存在大量包括抗氧化、金属蛋白酶等在内的可能与水母毒素生物学活性相关的编码序列。在此基础上，本项目拟对该转录组信息进行深入分析，明确触手转录谱及其转录产物之间关系，为挖掘、筛选及深入研究水母毒素蛋白及其他活性因子提供基础数据；同时，结合前期触手提取物抗氧化、蛋白水解等活性研究结果，重点关注转录组中抗氧化、astacin-like金属蛋白酶家族的编码序列，通过RACE-PCR、毕赤酵母表达系统表达及纯化等手段获得目标活性蛋白，开展其相关活性和功能研究，为阐明发形霞水母抗氧化、毒素胞外基质降解及扩散等作用的物质基础和机制提供科学依据。

中文关键词： 发形霞水母；转录组；毒素；抗氧化；astacin-like金属蛋白酶

英文摘要： Jellyfish, an abundant marine life, has the advantage of diverse biological activities. Many studies have proved jellyfish to be a potential resource pool for marine drugs which need in-depth research and development. In our previous study, by high-throughput sequencing, we have obtained the transcriptome of the tentacle of Cyanea capillata, a representative toxic jellyfish in Southeast China Sea. A preliminary analysis has shown that there are a large number of coding sequences for many possible bioactive factors including antioxidants, metalloproteases. On this basis, this study aims to make a further in-depth and integrated analysis of this transcriptome, understand the transcription profiling and the relationship between the transcripts,which can provide basic essential data for following screening, identification and study of jellyfish toxin and other important bioactive components. In addition, on the basis of previous studies on antioxidation and protein hydrolysis effects of tentacle-only extract from the Cyanea capillata, we will focus on the sequences in the transcriptome coding antioxidants and an astacin-like metalloprotease family in this study. By RACE-PCR, pichia pastoris yeast expression system and protein purification, we will obtain the target proteins and make a study on their activity detecti

英文关键词： Cyanea capillata；transcriptome；toxin；antioxidant；astacin-like metalloproteinase