基于脂质组成结构特异性的不同种属肉骨粉指纹光谱鉴别方法

项目名称： 基于脂质组成结构特异性的不同种属肉骨粉指纹光谱鉴别方法

项目编号： No.31201827

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 畜牧学与草地科学、兽医学、水产学

项目作者： 刘贤

作者单位： 中国农业大学

项目金额： 22万元

中文摘要： 针对为了高效防范疯牛病、适应饲料质量安全监管发展需求而提出的不同种属肉骨粉鉴别分析问题，提出一种新的思路和技术路线。在大量可靠样本和前期研究基础上，第一次从不同种属肉骨粉的脂质组成入手，研究挖掘不同种属肉骨粉原料和猪鸡肉骨粉中掺加牛羊源成分混合样品的脂质组成与结构特异性；研究上述不同脂质样品的红外与拉曼指纹光谱特征与差异，解析脂质特异性同时在两种指纹光谱特性上的表征与信息贡献，为阐明检测机理、建立科学可靠鉴别分析方法提供理论基础；优化选取不同种属肉骨粉鉴别分析的脂质特性变量与指纹光谱特征参数，研究建立基于脂质特异性和指纹光谱的不同种属肉骨粉原料、肉骨粉中牛羊源成分的兼顾检测精度和检测效率的鉴别分析方法。该方法可望与国际现行的动物源性饲料显微镜标准检测方法进行有效联用，对于有力保障禁止种内循环和同类相食的动物源性饲料监管措施，促进饲料产业的稳定和可持续发展，具有重要的科学意义和实际应用价值。

中文关键词： 肉骨粉；脂质；红外光谱；拉曼光谱；鉴别分析

英文摘要： This study is on the discrimination of different animal-originated meat and bone meal (MBM) owing to the BSE risk and development of legislation. A new idea and technical route is advanced. Based on a large amount of reliable samples and early research foundation, lipid characteristics, fingerprint spectroscopy (Infrared and Raman spectroscopy) characteristics of different animal-originated MBM materials, MBM adulterated with ruminant constituents will be studied. Superficial and internal connections between lipid characteristics and fingerprint spectroscopy will be explored by mathematics and chemometrics, which would give the theoretical interpretation for the discrimination of different animal-originated MBM by fingerprint spectroscopy. Optimized parameters of lipid characteristics and fingerprint spectroscopy characteristics would be then selected, and the following discrimination methods and models will be established: discrimination of different animal-originated MBM materials, discrimination of ruminant constituents in MBM samples. This method is hope to be in conjunction with the standard microscopy method effectively, and this study would bring into practice of species-specific legislation of animal-originated feedstuffs, and be of great scientific and practical importance for stability and sustainabili

英文关键词： Meat and bone meal；Lipid；Infrared spectroscopy；Raman spectroscopy；Discrimination