云南宣威肺癌潜在分子标志物的高通量多维组学筛选及功能机制研究

项目名称： 云南宣威肺癌潜在分子标志物的高通量多维组学筛选及功能机制研究

项目编号： No.81460325

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 段勇

作者单位： 昆明医科大学

项目金额： 47万元

中文摘要： 宣威肺癌是云南特有的、极具特点的一种肺癌，其不断上升的死亡率迫切需要尽快发掘与之诊疗相关的标志物，然而，以往国内外的研究并未鉴定出相关标志物。我们的前期研究发现宣威肺癌中存在与其分期、分化、发病等可能相关的基因组拷贝数变异和差异表达基因，并初步显示两者之间存在一定的规律性及内在联系。基于宣威肺癌鲜明的发病特点、既往研究的局限性及我们前期的相关研究，我们提出宣威肺癌在发生发展过程中的不同阶段存在不同的特征性分子标志物，宣威肺癌存在与其他肺癌不同的致病基因的假设。为此，我们拟同时应用array-CGH和基因表达谱芯片等新技术进一步在宣威肺癌样本中筛查标志物，并通过生物信息学分析、整合分析、分组与综合分析、基因功能研究、临床性能评价等，筛选和鉴定出能用于宣威肺癌诊断、分期、分化、转移、疗效及预后判断的潜在分子标志物和致病基因，探讨宣威肺癌的发病机制，为宣威肺癌的分子诊断和有效治疗奠定基础。

中文关键词： 宣威肺癌；标志物；微阵列比较基因组杂交；基因表达谱；整合分析

英文摘要： Xuanwei lung cancer, which only occurs in Yunnan province, has distinct characteristics, its rising mortality needs to explore effective markers for diagnosis and treatment as soon as possible, however, previous domestic studies have not identified the relevant markers. Our preliminary study detected large amounts of genomic copy number variation (CNVs) and differentially expressed genes associated with staging, differention and occurrence in Xuanwei lung cancer, and showed there may be certain regularity and intrinsic relation between CNVs and differentially expressed genes. Based on the distinctive characteristics of Xuanwei lung cancer, limitations of previous studies and our preliminary research, we propose the hypothesis that distinct molecular markers may be presented in different development stages of Xuanwei lung cancer, and the pathogenic genes presented in Xuanwei lung cancer may be different from that in other lung cancer. Thus, we intend to further perform genome-wide screening on Xuanwei lung cancer using array-CGH and gene expression profiling microarrays to detect molecular markers, and bioinformatic analysis, meta-analysis, integrative analysis, grouping and comprehensive analysis, gene function studies, clinical performance evaluation etc. will be carried out to screen out and identify potential molecular markers associated with diagnosis, staging, differention, metastasis, treatment and prognosis of Xuanwei lung cancer and candidate pathogenic genes, to explore the pathogenesis of Xuanwei lung cancer, and to lay the foundation for the molecular diagnosis and effective treatment of Xuanwei lung cancer.

英文关键词： Xuanwei lung caner;markers;array-CGH;gene expression profiling;integrative analysis